| Accession | CRX027031 |
| Organism | Canis lupus familiaris |
| Title | FAMIKEN00001.EXP1 |
| BioProject | PRJCA000879 |
| BioSample | SAMC036716 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
We used a QIAGEN DNeasy Blood & Tissue kit to extract genomic DNA and sheared to fragments randomly with the Covaris system. Then the libraries were constructed through end-repair, A-tailing, adapter ligation, and purification using the NEBNext Ultra DNA Library Prep Kit for Illumina. The concentrations of the libraries were measured using Qubit2.0. The libraries were diluted to 1.5ng/ul. Then the insert size was checked using Agilent 2100 and the purity was determined using Q-PCR method. Finally, it was sequenced on Illumina HiSeq platform. |
WGS |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 325
|
| Release date | 2019-07-25 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR030083 |
CRR030083_f1.fq.gz
CRR030083_r2.fq.gz
|
1,121.73
1,249.31
|
| CRR030084 |
CRR030084_f1.fq.gz
CRR030084_r2.fq.gz
|
5,484.29
6,138.39
|
| CRR059190 |
CRR059190_f1.fq.gz
CRR059190_r2.fq.gz
|
3,940.02
4,434.52
|
|
| Submitter | Bo-wen Zhou (zhoubowen@mail.kiz.ac.cn) |
|---|
| Organization | Kunming Institute of Zoology, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-05-21 |
|---|
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