Experiment information
Accession CRX025645
Organism metagenome
Title SYLQ4
BioProject PRJCA000797
BioSample SAMC027085
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total genome DNA was extracted using QIAamp Fast DNA Stool Mini Kit (QIAGEN). The V4 region of 16S rDNA was amplified by using the 515f/806r primer set with Phusion® High-Fidelity PCR Master Mix (NEB). PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN). Then, libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina)and sequenced by Illumina HiSeq2500 platform. AMPLICON METAGENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 300
Release date2020-03-23
Run
Run accession Run data file information
File nameFile size (MB)
CRR028512 CRR028512_f1.fq.gz
CRR028512_r2.fq.gz
9.48
9.06
SubmitterHai Xiang (vamyluo@126.com)
OrganizationChina Agricultural University
Date submitted2018-03-27