Accession | CRX025608 |
Organism | metagenome |
Title | LSY7 |
BioProject | PRJCA000797 |
BioSample | SAMC027048 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total genome DNA was extracted using QIAamp Fast DNA Stool Mini Kit (QIAGEN). The V4 region of 16S rDNA was amplified by using the 515f/806r primer set with Phusion® High-Fidelity PCR Master Mix (NEB). PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN). Then, libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina)and sequenced by Illumina HiSeq2500 platform. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 300
|
Release date | 2020-03-23 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR028475 |
CRR028475_f1.fq.gz
CRR028475_r2.fq.gz
|
10.12
9.84
|
|
Submitter | Hai Xiang (vamyluo@126.com) |
Organization | China Agricultural University |
Date submitted | 2018-03-27 |