| Accession | CRX025594 |
| Organism | metagenome |
| Title | LSW3 |
| BioProject | PRJCA000797 |
| BioSample | SAMC027034 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total genome DNA was extracted using QIAamp Fast DNA Stool Mini Kit (QIAGEN). The V4 region of 16S rDNA was amplified by using the 515f/806r primer set with Phusion® High-Fidelity PCR Master Mix (NEB). PCR products were purified using the QIAquick Gel Extraction Kit (QIAGEN). Then, libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina)and sequenced by Illumina HiSeq2500 platform. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 300
|
| Release date | 2020-03-23 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR028461 |
CRR028461_f1.fq.gz
CRR028461_r2.fq.gz
|
11.09
10.66
|
|
| Submitter | Hai Xiang (vamyluo@126.com) |
|---|
| Organization | China Agricultural University |
|---|
| Date submitted | 2018-03-27 |
|---|
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