Accession | CRX022321 |
Organism | human gut metagenome |
Title | Healthy cohort sample 39 gut microbiota |
BioProject | PRJCA000673 |
BioSample | SAMC019295 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A total of 200mg of each fecal sample was used for DNA extraction, using PowerSoil DNA kit (MoBio, USA) according to the manufacturer’s instructions. The KAPA HiFiHotStartReadyMix (KAPA, USA) was used to amplify the 16S rRNA (V3-V4) gene tags. Amplicons |
OTHER |
METAGENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 481
|
Release date | 2018-06-01 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR024625 |
CRR024625_f1.fq.gz
CRR024625_r2.fq.gz
|
4.94
5.87
|
|
Submitter | Jintian Xu (xujintian@big.ac.cn) |
Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
Date submitted | 2017-12-20 |