Experiment information
Accession CRX020533
Organism Mus
Title PDGF induced CM proliferation and regeneration
BioProject PRJCA000636
BioSample SAMC017384
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
NULL-1 CMs were isolated from P1 neonatal hearts of PdgfrbD849Vfl/fl mice by using the neonatal dissociation kit and isolation kit (gentleMACS) .For the virus infection experiments, CMs were infected with 300 MOI of Ad-CMV-Cre or Ad-CMV-Null (Hanbio, ShangHai, China) in DMEM/F12 medium.48 hours later, CMs were harvested for RNA-seq RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2017-11-19
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR022669 2017-11-19 GFP1_R1.fastq.gz
GFP1_R2.fastq.gz
3,522.44
3,885.38
SubmitterYUE Zhang (yuegaoxin1120@163.com)
OrganizationsFuwai Hospital, Chinese Academy of Medical Sciences
Date submitted2017-11-16
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Experiments(6)