Experiment information
Accession CRX036346
Organism Populus trichocarpa
Title four stress treatment
BioProject PRJCA001161
BioSample SAMC051297
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
osmotic3 P. trichocarpa plants were grown in a greenhouse under a 16 h light/8 h dark photoperiod, with light provided by cool white fluorescent lights (250 μmol m-2 s-1 PPFD). For stress treatments, 12 plants obtained from a single genotype were used for chilling stress (three plants; 4°C, 6 h, 250 μmol m-2 s-1 PPFD),for heat stress (three plants; 42°C, 6 h, 250 μmol m-2 s-1 PPFD), three were exposed to 150 mM NaCl, 30% polyethylene glycol (PEG) 6000 for 6 h, and three were exposed to a drought treatment (25°C, 250 μmol m-2 s-1 PPFD, soil moisture contents 15-20%). Leaves were collected from P. trichocarpa for RNA extraction with different treatment (three biological replicates per treatment). RNA-Seq TRANSCRIPTOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
Release date2018-12-14
Run
Run accession Release date Run data file information
File nameFile size (MB)
CRR040915 2018-12-14 salt3_1.clean.fq.gz
salt3_2.clean.fq.gz
1,450.37
1,512.83
Submitterxie jianbo (xiejianbo1860@163.com)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2018-12-12