| Accession | CRX019693 |
| Organism | Homo sapiens |
| Title | RNA-Seq of CSC |
| BioProject | PRJCA000539 |
| BioSample | SAMC016541 |
| Platform | Illumina HiSeq 2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA of sorted CSCs was extracted using Trizol reagent and mRNA was enriched using oligo (dT) magnetic beads, approximately 1 ug of mRNA was fragmented. Then these fragments of about 200-250 bp were separated by electrophoresis. After that, cDNA fragments were synthesised using them through end repair, adenylation of the 3' ends and adapter ligation. Finally, those ranging from 250-300 bp were excised from electrophoretic gels for sequencing using an Illumina HiSeq2000 system. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 250
|
| Release date | 2019-09-04 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR021468 |
CRR021468_f1.fastq.gz
CRR021468_r2.fastq.gz
|
1,971.3
1,992.33
|
|
| Submitter | Ke An (anke@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2017-09-04 |
|---|
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