To identify the m6A modification changes in cellular mRNAs following EBV infection, we performed methylated RNA immunoprecipitation and sequencing (MeRIP-seq) of infected versus uninfected nasopharyngeal epithelial cells (Fig. 1A). After extracting the mRNA from NP460 and HK1 cells at 24h post-infected with EBV, we used an anti-m6A antibody to enrich m6A-modified RNA fragments prior to RNA sequencing of both the input and immunoprecipitated fractions for MeRIP-seq.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
Organization: Sun Yat-sen University Cancer Center
Submission date: 2021-02-18
Aspera Command Line
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.
Note: HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client) or Asepra Command Line.
HRA000681.xlsx