Here, we characterized the gene expression profile in tolerant T cells which was induced in healthy donors by granulocyte colony-stimulating factor, a stem cell mobilizer extensively used in HSCT. We identified suppressor of cytokine signaling 1 (SOCS1) as an essential immune checkpoint for T cell tolerance in the mouse models and primary T cells in the HSCT context. Further spatial multiomics analysis characterized the distinct three-dimensional genome architecture and the gene regulatory network in tolerant T cells. We found STAT3 competes with CTCF and mediates the formation of a new chromatin loop between the SOCS1 promoter and upstream super enhancers during the induction of T cell tolerance. This study identifies SOCS1 as a key immune checkpoint and potential immune target for improving outcome of patients with HSCT.
For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.
You need to install the Aspera Connect plugin before uploading your files. If you dont have Aspera Connect plugin installed in your computer, please Download Aspera Connectand install it first.
[path/to/ascp]: Microsoft Windows: C:\Program Files\Aspera\Aspera Connect\bin\ascp.exe or
c:\users\[username]\AppData\Local\Programs\Aspera\Aspera Connect\bin\ascp.exe
Mac OS X: /Applications/Aspera/Connect.app/Contents/Resources/ascp (for admin's installation) or
/Users/[username]/Applications/Aspera/Connect.app/Contents/Resources/ascp (for non-admin's installation)
Linux: /opt/aspera/bin/ascp or
/home/[username]/aspera/connect/bin/ascp
[path/to/key/file] must be an absolute path, e.g.: /home/keys/aspera.openssh
[path/to/folder/containing/files] needs to specify the local folder that contains all the files to upload.