NGDC - GSA for Human

HRA000313

HRA000313.xlsx

Title:

Reclassification of classic T cell subsets and identification of novel cell types by single-cell RNA sequencing

Release date:

2022-01-01

Description:

Single cell RNA sequencing (scRNA-seq) clusters cells using genome-wide gene expression profiles. The relationships between scRNA-seq Clustered-Populations (scCPops) and cell surface molecules defined classic T cell subsets are unclear. Here,we interrogated 6 beads-enriched T cell subsets with 62,235 single cell transcriptomes and re-grouped them into 9 scCPops. Beads-enriched CD4 Naive, CD8 Naive and CD4 memory were mainly clustered into their scCPop counterparts, while the other T cell subsets were clustered into multiple scCPops including unexpected mucosal-associated invariant T cell and natural killer T cell. Most interestingly, we discovered a new T cell type that highly expressed Interferon Signaling Associated Genes (ISAGs), namelyIFNhi T. We further enriched IFNhi T for scRNA-seq analyses. IFNhi T cluster disappeared on tSNE after removing ISAGs, and IFNhi T cluster showed up by tSNE analyses of ISAGs alone, indicating ISAGs are the major contributor of IFNhi T cluster.

Data Accessibility:

Controlled access Request Data

BioProject:

PRJCA003391

Study type:

healthy human cell type

Data Access Committee

For each controlled access study, there is a corresponding Data Access Committee(DAC) to determine the access permissions. Access to actual data files is not managed by NGDC.

DAC NO.:

HDAC000042

DAC name:

SUSTECH_SCPM

Contact person:

Jin Wenfei Jin

Email:

jinwf@sustech.edu.cn

Description:

SIngle-cell Sequencing in Precison Medicine

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