Database Commons

a catalog of biological databases

e.g., animal; RNA; Methylation; China

Database information

ICG (A knowledgebase of Internal Control Genes for RT-qPCR normalization)

General information

Description: ICG is a wiki-based knowledgebase of internal control genes (or reference genes) for RT-qPCR normalization in a variety of species. Based on community curation, ICG harnesses collective intelligence to integrate a comprehensive collection of internal control genes curated from a large volume of literatures and provides appropriate internal control genes corresponding to specific experimental conditions for both model and non-model organisms.
Year founded: 2017
Last update: 2017-10-03
Version: 1.0
Accessibility:
Manual:
Accessible
Real time : Checking...
Country/Region: China
Data type:
Data object:
Database category:
Major organism:
Keywords:

Contact information

University/Institution: Beijing Institute of Genomics, Chinese Academy of Sciences
Address: 1 Beichen West Road, Chaoyang District, Beijing 100101, China
City: Beijing
Province/State:
Country/Region: China
Contact name (PI/Team): Zhang Zhang
Contact email (PI/Helpdesk): zhangzhang@big.ac.cn

Record metadata

Created on: 2017-11-05
Curated by:
Zhang Zhang [2018-11-28]
Lina Ma [2018-05-25]
Dong Zou [2018-03-02]
Dong Zou [2018-01-25]
Lin Liu [2017-11-06]
Lin Liu [2017-11-05]

Ranking

All databases:
1156/4549 (74.61%)
Expression:
210/800 (73.875%)
Literature:
69/284 (76.056%)
1156
Total Rank
11
Citations
5.5
z-index

Community reviews

5 Stars (1)
Data quality & quantity:
Content organization & presentation
System accessibility & reliability:

Word cloud

Publications

29036693
ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization. [PMID: 29036693]
Sang J, Wang Z, Li M, Cao J, Niu G, Xia L, Zou D, Wang F, Xu X, Han X, Fan J, Yang Y, Zuo W, Zhang Y, Zhao W, Bao Y, Xiao J, Hu S, Hao L, Zhang Z.

Real-time quantitative PCR (RT-qPCR) has become a widely used method for accurate expression profiling of targeted mRNA and ncRNA. Selection of appropriate internal control genes for RT-qPCR normalization is an elementary prerequisite for reliable expression measurement. Here, we present ICG (http://icg.big.ac.cn), a wiki-driven knowledgebase for community curation of experimentally validated internal control genes as well as their associated experimental conditions. Unlike extant related databases that focus on qPCR primers in model organisms (mainly human and mouse), ICG features harnessing collective intelligence in community integration of internal control genes for a variety of species. Specifically, it integrates a comprehensive collection of more than 750 internal control genes for 73 animals, 115 plants, 12 fungi and 9 bacteria, and incorporates detailed information on recommended application scenarios corresponding to specific experimental conditions, which, collectively, are of great help for researchers to adopt appropriate internal control genes for their own experiments. Taken together, ICG serves as a publicly editable and open-content encyclopaedia of internal control genes and accordingly bears broad utility for reliable RT-qPCR normalization and gene expression characterization in both model and non-model organisms. ? The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Nucleic Acids Res. 2018:46(D1) | 11 Citations (from Europe PMC, 2020-04-04)