Accession PRJCA002144
Title RNA-seq of DNMT3A KO HEK293T cells
Relevance Medical
Data types Transcriptome or Gene expression
Organisms Homo sapiens
Description To reveal the general effect of DNMT3A and its regulated genes profile, we knocked out the gene functionally by inducing framework mutations via CRISPR/Cas9 technology. Both WT and KO cell lines were sequenced for transcripsome. Significant gene expression regulation in pathways related to cell metabolism and proliferation processes was observed, which could explain alterations in cell growth rate of DNMT3A deficient cells, at least in part.
Sample scope Monoisolate
Release date 2020-01-15
Publication
PubMed ID Article title Journal name DOI Year
31845522 Identification of differentially expressed genes by single-cell transcriptional profiling of umbilical cord and synovial fluid mesenchymal stem cells Journal of Cellular and Molecular Medicine 10.1111/jcmm.14891 2019
Biomaterial provider Dr. Huili Lu
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) General Program 81273576
Science and Technology Commission of Shanghai Municipality 17431904500
Submitter Huili    Lu  (roadeer@sjtu.edu.cn)
Organization Shanghai Jiao Tong University
Submission date 2020-01-15

Project Data

Resource name Description
BioSample (6)  show -
GSA (1) -
CRA002294 RNA-seq of DNMT3A KO HEK293T cells