Accession

PRJCA001267

Title

An Arabidopsis secondary metabolite targets bacterial virulence for defense

Relevance

Model organism

Data types

Metagenome

Organisms

Microbiota
Pseudomonas syringae

Description

Plants deploy a variety of seconday metabolites to fend off pathogen attack. While it is generally believed that defense compounds are toxic or inhibitory to microbes, the exact mechanisms are often unknown. Here we show that Arabidopsis plants produce compounds that selectively inhibit Pseudomonas syringae virulence gene expression, rather than inhibit bacterial growth or viability. Through bioassay-guided purification, structural elucidation, and chemical synthesis, we demonstrate that one such compound is sulforaphane, a derivative of aliphatic glucosinolate. At physiological concentrations, SFN preferentially inhibits the expression of P. syringae genes encoding the type III secretion system in vitro and in planta. Plants defective in SFN production is unable to attenuate bacterial type III gene expression and exhibit increased susceptibility to P. syringae bacteria. The selectivity of SFN to P. syringae virulence is further supported by the findings that in Arabidopsis plants SFN does not impact a P. syringae mutant lacking the type III system and leaf-associated bacterial microbiota. We further performed target profiling and mutagenesis analyses to show that SFN attenuates P. syringae virulence by specifically targeting Cys209 of HrpS, a key transcription factor controlling type III gene expression, and a mutation in Cys209 renders the bacterium insensitive to SFN.

Sample scope

Multispecies

Release date

2019-02-02

Grants

Agency	program	Grant ID	Grant title
No funding support

Submitter

Yong-Xin Liu (woodcorpse@163.com)

Organization

Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Submission date

2019-02-02

Project Data

Resource name	Description
BioSample (57) show	-
SAMC134105	SFN treatment sax bacteria SNF_3
SAMC134104	SFN treatment sax bacteria SNF_2
SAMC134103	SFN treatment sax bacteria SNF_1
SAMC134102	DMSO treatment sax bacteria mock_3
SAMC134101	DMSO treatment sax bacteria mock_2
SAMC134100	DMSO treatment sax bacteria mock_1
SAMC072601	SFN treatment SNF_3
SAMC072600	SFN treatment SNF_2
SAMC072599	SFN treatment SNF_1
SAMC072598	DMSO treatment mock_3
SAMC072597	DMSO treatment mock_2
SAMC072596	DMSO treatment mock_1
SAMC072595	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R12
SAMC072594	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R11
SAMC072593	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R10
SAMC072592	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R09
SAMC072591	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R08
SAMC072590	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R07
SAMC072589	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R06
SAMC072588	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R05
SAMC072587	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R04
SAMC072586	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R03
SAMC072585	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R02
SAMC072584	16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R01
SAMC072583	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R11
SAMC072582	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R10
SAMC072581	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R08
SAMC072580	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R07
SAMC072579	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R06
SAMC072578	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R05
SAMC072577	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R04
SAMC072576	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R03
SAMC072575	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R02
SAMC072574	16S amplicon sequencing of plant phyllosphere microbiome sampleColPhyl2R01
SAMC072573	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R12
SAMC072572	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R11
SAMC072571	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R09
SAMC072570	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R08
SAMC072569	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R07
SAMC072568	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R06
SAMC072567	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R05
SAMC072566	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R04
SAMC072565	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R03
SAMC072564	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R02
SAMC072563	16S amplicon sequencing of plant leaf microbiome sampleMybTot1R01
SAMC072562	16S amplicon sequencing of plant leaf microbiome sampleColTot1R12
SAMC072561	16S amplicon sequencing of plant leaf microbiome sampleColTot1R11
SAMC072560	16S amplicon sequencing of plant leaf microbiome sampleColTot1R10
SAMC072559	16S amplicon sequencing of plant leaf microbiome sampleColTot1R09
SAMC072558	16S amplicon sequencing of plant leaf microbiome sampleColTot1R08
SAMC072557	16S amplicon sequencing of plant leaf microbiome sampleColTot1R07
SAMC072556	16S amplicon sequencing of plant leaf microbiome sampleColTot1R06
SAMC072555	16S amplicon sequencing of plant leaf microbiome sampleColTot1R05
SAMC072554	16S amplicon sequencing of plant leaf microbiome sampleColTot1R04
SAMC072553	16S amplicon sequencing of plant leaf microbiome sampleColTot1R03
SAMC072552	16S amplicon sequencing of plant leaf microbiome sample ColTot1R02
SAMC072551	16S amplicon sequencing of plant leaf microbiome sample ColTot1R01
GSA (3)	-
CRA002319	Pseudomonas RNA-Seq data
CRA001772	Pseudomonas RNA-Seq data
CRA001771	Arabidopsis leaf microbiota 16S amplicon data