Accession

PRJCA001057

Title

Discovery of unique single nucleotide polymorphisms associated with high nighttime temperature stress in rice using a hybrid sequencing strategy

Relevance

Agricultural

Data types

Raw sequence reads

Organisms

Oryza sativa

Description

First, comparative transcriptomics between a heat-tolerant strain and heat-sensitive strain in response to high nighttime temperature stress was carried out using a hybrid sequencing strategy (TGS and SGS) to detect the relative differentially expressed genes (RDEGs). Second, the genomic homologous DNA sequences of the RDEGs were retrieved from the reference genome. Third, genome re-sequencing for both HTS and HSS was performed, and the generated short reads were used to call unique SNP loci for the RDEGs, using the retrieved homologous DNA sequences as a reference.

Sample scope

Monoisolate

Release date

2018-10-17

Publication

PubMed ID	Article title	Journal name	DOI	Year
	Discovery of unique single nucleotide polymorphisms in rice in response to high nighttime temperature stress using a hybrid sequencing strategy	Environmental and Experimental Botany	10.1016/j.envexpbot.2019.02.005	2019
	Comparative Analysis of the Transcriptional Response of Tolerant and Sensitive Wheat Genotypes to Drought Stress in Field Conditions	Agronomy	10.3390/agronomy8110247	2018

Grants

Agency	program	Grant ID	Grant title
National Natural Science Foundation of China (NSFC)		31471467
National Natural Science Foundation of China (NSFC)		31560387
National Natural Science Foundation of China (NSFC)		31860359

Submitter

Liao Jiang-Lin (jlliao514815@163.com)

Organization

Jiangxi Agricultural University

Submission date

2018-10-07

Project Data

Resource name	Description
BioSample (15) show	-
SAMC046979	SGS for the genome re-sequencing of the heat-sensitive strain
SAMC046978	SGS for the genome re-sequencing of the heat-tolerant strain
SAMC046977	SGS for the transcriptome of the heat-sensitive strain of the control 3
SAMC046976	SGS for the transcriptome of the heat-sensitive strain of the control 2
SAMC046975	SGS for the transcriptome of the heat-sensitive strain of the control 1
SAMC046974	SGS for the transcriptome of the heat-sensitive strain of the treatment 3
SAMC046973	SGS for the transcriptome of the heat-sensitive strain of the treatment 2
SAMC046972	SGS for the transcriptome of the heat-sensitive strain of the treatment 1
SAMC046971	SGS for the transcriptome of the heat-tolerant strain of the control 3
SAMC046970	SGS for the transcriptome of the heat-tolerant strain of the control 2
SAMC046969	SGS for the transcriptome of the heat-tolerant strain of the control 1
SAMC046968	SGS for the transcriptome of the heat-tolerant strain of the treatment 3
SAMC046967	SGS for the transcriptome of the heat-tolerant strain of the treatment 2
SAMC046966	SGS for the transcriptome of the heat-tolerant strain of the treatment 1
SAMC046965	TGS for the full-length mRNA sequences
GSA (1)	-
CRA001148	Discovery of unique single nucleotide polymorphisms associated with high nighttime temperature stress in rice using a hybrid sequencing strategy