| Accession |
PRJCA000979 |
| Title |
Single-cell RNA-seq of HeLa cells |
| Relevance |
Evolution |
| Data types |
Transcriptome or Gene expression
Raw sequence reads
|
| Organisms |
Homo sapiens
|
| Description |
DNA replication causes the dosage imbalance in every cell cycle. Although acetylated histones (H3K56ac) can incorporate into newly replicated DNA regions and suppress their expression, this compensatory mechanism cannot completely restore the dosage balance. In this study, we used single-cell transcriptome analyses to estimate the extent of dosage compensation between early- and late-replicating genes in mammalian cells. We found a global increase in expression level of early-replicating genes during the mid-S phase, resulting in a dosage imbalance between early- and late-replicating genes in HeLa cells. We proposed a synchronized replication hypothesis that genes encoding subunits of the same protein complex are replicated simultaneously so that the dosage balance within a protein complex is warranted. Our study reveals that the demand for dosage balance during S phase plays an important role in the optimization of the replication-timing program. |
| Sample scope |
Single cell |
| Release date |
2019-09-22 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 31662304
|
Synchronized replication of genes encoding the same protein complex in fast-proliferating cells
|
Genome Research
|
10.1101/gr.254342.119
|
2019
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
91731302
|
|
|
|
| Submitter |
ying
chen (ychen@genetics.ac.cn)
|
| Organization |
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
| Submission date |
2018-08-09 |
