| Accession | PRJCA000910 |
|---|---|
| Title | Somatic Mutation of Normal Intestinal Crypt |
| Relevance | Evolution |
| Data types |
Whole genome sequencing
Variation |
| Organisms | Homo sapiens |
| Description | We cultured organoids from single crypts collected from human small intestine (SmI) and colon (CO). Note that a crypt of either tissue is usually assumed to be the unit of evolution, within which competitions between cells happen. In that case, the size of the stem cell population in a crypt (N) would determine the efficacy of selection. An added benefit of whole-crypt cloning is that DNA sequences so obtained could provide some information on the effective size of the stem cell population in a crypt which will be important for determining the efficacy of selection. The results suggest that cancer risk may in fact depend primarily on the efficacy of natural selection, as illustrated by the >50 fold difference in cancer risk between the small intestine and the colon. |
| Sample scope | Monoisolate |
| Release date | 2018-09-30 |
| Biomaterial provider | Department of Pathology, Keck School of Medicine of the University of Southern California |
| Submitter | Zongkun Shi (shizongkun@gmail.com) |
| Organization | Sun Yat-sen University |
| Submission date | 2018-06-14 |