Accession PRJCA000841
Title Methylation Changes in Soybean Early Maturation Seed Compartments Using Laser Capture Microdissection (LCM)
Relevance Agricultural
Data types Epigenomics
Organisms Glycine max
Description What methylation changes are occurring in different compartments of early maturation stage seed largely remains unknown. To uncover the possible role of DNA methylation in different compartments of early maturation stage seed, we characterized the methylome of two major compartments (abaxial parenchyma and adaxial parenchyma) in embryonic cotyledon, four major compartments (parenchyma, plumule, root tip, and vascular) in embryonic axis, and seed coat layers (parenchyma and palisade) using Illumina sequencing. The whole seed coat and embryonic cotyledons were captured using LCM to serve as controls. Overall design: Illumina sequencing of bisulfite-converted genomic DNA from cotyledon abaxial parenchyma (EM-COT-ABPY), cotyledon adaxial parenchyma (EM-COT-ADPY), seed coat parenchyma (EM-SC-PY), seed coat palisade (EM-SC-PA), axis parenchyma (EM-AX-PY), axis plumule (EM-AX-PL), axis root tip (EM-AX-RT), axis vascular (EM-AX-VS), whole seed coat (EM-SC), and whole embryonic cotyledons (EX-COT).
Sample scope Multiisolate
Release date 2018-06-30
Accessions in other database
Accession Database name
PRJNA165675 NCBI
Submitter Fang    Liang  (liangf@big.ac.cn)
Organization Beijing Institute of Genomics, Chinese Academy of Sciences
Submission date 2018-04-25

Project Data

Resource name Description
BioSample (2) -
SAMC043640 early maturation-stage seed coat parenchyma
SAMC043639 early maturation-stage seed coat palisade