Accession |
PRJCA000432 |
Title |
Hypoxia-activated signaling pathways contribute to scleral myofibroblast differentiation and myopia development |
Relevance |
Model organism |
Data types |
Transcriptome or Gene expression
|
Organisms |
|
Description |
Scleral fibroblasts/myofibroblast play important roles in extracellular matrix remodeling during myopia development. However, it is with uncertainty how myopia-induced vision bluring from retina triggers sclera ECM remodeling and whether or not changes in myofibroblast function underlie myopia progression. In this study, we identified gene expression changes associated with increase in myofibroblast population during myopia development. The proportion of myofibroblast-like cells (A2) increased in T eyes, suggesting a phenotypic shift towards A2 cells mediated by enhanced differentiation of fibroblasts (A1) into myofibroblasts. The myofibroblasts differentiated during myopia express lower levels of collagen. This shift was mimicked by exposing fibroblasts to two different in vitro hypoxic models. Pathway analysis revealed that the major functional differences between these two subpopulations arise from activation of eIF2 signaling, mTOR signaling and hypoxia signaling in cardiovascular system which were further validated in myopia animal models. Our results indicate that myopia development induces differentiation of myofibroblasts that may be affected by scleral hypoxic condition. It provides strategies to control myopia progression at an early stage via regulating scleral microenvironment. |
Sample scope |
Single cell |
Release date |
2017-04-24 |
Submitter |
Lili
Deng (denglili@big.ac.cn)
|
Organization |
Beijing Institute of Genomics, Chinese Academy of Sciences |
Submission date |
2017-04-24 |