Accession | PRJCA000352 | ||||||||||
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Title | hMeDIP_seq of vitamin C-treated kidney tumour cells | ||||||||||
Relevance | Medical | ||||||||||
Data types | Epigenomics | ||||||||||
Organisms | Homo sapiens | ||||||||||
Description | Recent study has revealed that 5-hydroxymethylcytosine (5hmC) is globally lost in virtually all ccRCC (clear cell renal cell carcinoma) tumour tissues and served as an independent prognostic marker for ccRCC. Thus, re- establishment of 5hmC levels in ccRCC tumour cells may have therapeutic effect. Here, we use vitamin C(Sodium L-ascorbate,AsA-Na) and its derivative L-Ascorbic acid 2-phosphate sesquimagnesium(APM) to restore 5hmC levels in 786O and A498 cells,786O xenografts and also in a ccRCC patient primary tumor or normal cells. The hMeDIP_seq was used to determine the genome-wide 5hmC reprogramming pattern in these samples. We also performed RNA seq of control or vitamin C-treated 786O cells. | ||||||||||
Sample scope | Monoisolate | ||||||||||
Release date | 2020-02-13 | ||||||||||
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Submitter | Guangzhe Ge (geguangzhe@big.ac.cn) | ||||||||||
Organization | Beijing Institute of Genomics (BIG) | ||||||||||
Submission date | 2017-02-13 |