| Accession |
PRJCA000241 |
| Title |
3D Chromatin Structures of Mature Gametes and Structure Reprogramming duringMammalian Embryogenesis |
| Relevance |
Model organism |
| Data types |
Epigenomics
|
| Organisms |
Mus musculus
Homo sapiens
Mus musculus cytomegalovirus 2
|
| Description |
The dynamics of 3D chromatin structures during mammalian embryo development remains limited known. We report the 3D chromatin architectures of mouse gametes and early embryos using an optimized Hi-C method with low-cell samples. We find that the mature oocytes at metaphase II stage do not have topologically associated domains. In sperm, the extra-long range interactions (>4 mega bases) and interchromosomal interactions are more frequent. The high-order structures of both the paternal and maternal genomes in zygotes and 2-cell embryos are obscure. The chromatin structures are gradually re-established through development. We find that the unmethylatedCpGs are enriched in A compartment, and the decreased levels of CpG methylation in A compartment are higher than those in B compartment in embryos. Furthermore, the establishment of TAD structure requires DNA replication in 2-cell embryos, but not zygotic genome activation. In summary, the global reprogramming of the chromatin architecture occurs during early mammalian development. |
| Sample scope |
Multiisolate |
| Release date |
2017-07-13 |
| Publication |
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
National Key Technologies R&D Program
|
2018YFC1003300
|
|
|
|
| Submitter |
Xuepeng
Chen (chenxp@big.ac.cn)
|
| Organization |
BIG,CAS |
| Submission date |
2016-06-01 |
