| Accession |
PRJCA015743 |
| Title |
viral metagenome of piglets |
| Relevance |
Medical |
| Data types |
Genome sequencing
|
| Organisms |
Microvirus
|
| Description |
From April to May in spring 2018 for viral metagenomic analysis 89 fecal samples of diarrhea piglets were collected from farms in Jiangsu City China All piglets were under one month of age All samples were collected by disposable materials Fecal samples were resuspended with 10 volumes of phosphate buffered saline PBS and vigorously rotated for 5 minutes After centrifugation 10min 15000 g the supernatant was placed in a centrifuge tube 500 of the mixed supernatant was filtered through a microwell filter in order to remove particles such as bacteria and eukaryotes Fecal samples were treated at 37 for 90 minutes to digest free non-viral nucleic acids and the filtrate that had been enriched in virus particles was treated with a mixture of DNase and RNase A QIAamp Viral RNA Mini Kit QIAGEN was used to extract nucleic acid according to the manufacturer's instruction 89 libraries were then constructed using Nextera XT DNA Sample Preparation Kit Illumina and sequenced using the Illumina Miseq platform with 250 bases paired ends with double barcodes for each library For bioinformatics analysis paired-end reads of 250 bp generated by MiSeq were debarcoded using vendor software from Illumina |
| Sample scope |
Monoisolate |
| Release date |
2023-03-23 |
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2017YFC1200201
|
National Key Research and Development Programs of China for Virome in Important Wildlife
|
| Other
|
|
2020220456
|
Natural Science Foundation of the Jiangsu Higher Ediucation institutions of China
|
| Other
|
|
CARS-42-35
|
China Agriculture Research System of MOF and MARA
|
| Other
|
|
PG21171
|
Climbing Plan of Shanghai Academy of Agricultural Sciences
|
|
|
| Submitter |
Lingling
Qian (qll980096@126.com)
|
| Organization |
Jiangsu University |
| Submission date |
2023-03-23 |
