Accession PRJCA001042
Title Single-cell sequencing of holo-transcriptome
Relevance Single cell sequencing
Data types Exome
Transcriptome or Gene expression
Raw sequence reads
Organisms Mus musculus
Homo sapiens
Description We introduce a single-cell holo-transcriptome sequencing (Holo-Seq) has the same quantitative accuracy and uniform coverage with a complete strand of origin information as bulk RNA-seq. Most importantly, Holo-Seq can simultaneously observe small-RNAs and mRNAs in a single cell. Furthermore, we acquire small-RNA and mRNA dual transcriptomes of 32 human hepatocellular carcinoma single cells, which display the genome-wide super-enhancer activity and hepatic neoplasm kinetics of these cells.
Sample scope Single cell
Release date 2018-09-29
Publication
PubMed ID Article title Journal name DOI Year
30333049 Holo-Seq: single-cell sequencing of holo-transcriptome Genome Biology 10.1186/s13059-018-1553-7 2018
Grants
Agency program Grant ID Grant title
Nature Science Foundation of Zhejiang Province LR15C060001
Ministry of Science and Technology of the People's Republic of China (MOST) National Program on Key Research Project of China 2017YFA0104901
Submitter Zhengyun    Xiao  (xiaozhengyun1993@126.com)
Organization Zhejiang University
Submission date 2018-09-21

Project Data

Resource name Description
BioSample (152)  show -
GSA (2) -
CRA001131 Single-cell sequencing of holo-transcriptome: Mus musculus
CRA001133 Single-cell sequencing of holo-transcriptome: Homo sapiens