| Accession | PRJCA000378 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Title | Droplet-Cirseq | ||||||||||
| Relevance | Evolution | ||||||||||
| Data types | Whole genome sequencing | ||||||||||
| Organisms |
Enterobacteria phage phiX174 sensu lato
Escherichia coli |
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| Description | We developed a method namely Droplet-CirSeq to significantly improve the amplification bias, which presents obvious superiority over the currently prevalent methods in exploitation of ultra-low frequency mutations. Droplet-CirSeq would be promisingly used in the identification of low frequency mutations initiated from extremely low input DNA, such as DNA of uncultured microorganisms, captured DNA of target region, circulation DNA of plasma et al, and its creative conception of rolling circle amplification in droplets would also be used in other low input DNA amplification fields. | ||||||||||
| Sample scope | Monoisolate | ||||||||||
| Release date | 2017-03-20 | ||||||||||
| Publication |
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| Submitter | Kaile Wang (llee90504452@gmail.com) | ||||||||||
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences | ||||||||||
| Submission date | 2017-03-20 |
| Resource name | Description |
|---|---|
| BioSample (5) | - |
| SAMC012206 | Phix174 |
| SAMC012205 | E.coli-DH5a |
| SAMC012204 | E.coli-W3110 |
| SAMC012203 | E.coli mixture (W3110 and DH5a)-1_100 |
| SAMC012202 | E.coli mixture (W3110 and DH5a) |
| GSA (1) | - |
| CRA000212 | CRA_212 |