TADA - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: TADA
Synonym: ARABIDOPSIS THALIANA TRNA ADENOSINE DEAMINASE A, ATTADA, RNA ARGININE ADENOSINE DEAMINAS, TADA1, TRNA ARGININE ADENOSINE DEAMINASE
Description: Encodes the chloroplastic A-to-I tRNA editing enzyme.
Protein Family: Adenosine Deaminase
Subclass: NA
Construct Structure: Conserved Deaminase Domain
Gene ID & Species: AT1G68720 (Arabidopsis thaliana)
Edited Gene(s): trnR
Editing Type(s): A-to-I (5)
Publication(s): [1] Arabidopsis tRNA Adenosine Deaminase Arginine Edits the Wobble Nucleotide of Chloroplast tRNAArg(ACG) and Is Essential for Efficient Chloroplast Translation, The Plant Cell, 2009. [PMID=19602623]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G68720 Chloroplast trnR 34 Exon A-to-I ACG=>ICG NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
GK-119G08tada-1T-DNA InsertionT-DNA insertion is mapped to the first exon of the gene, 1597 nucleotides downstream of the initiation codonHomozygous; KnockoutSlow growth; Pale-green; Round leavesNANADirect Sequencing of PCR Products0.00%UneditedAbsent19602623
GK-119G08tada-1 + ΔN-TADAComplementationFuses the deaminase domain (DN-TADA) to the signal peptide of the chloroplast protein RECA1 (At1g79050) and expresses it in tada-1 under the control of the 35S promoterNANANANADirect Sequencing of PCR Products50.00%MediumRestored19602623
Agri-140-69-2-CATMA1a58100RNAi (Silencing)RNAiNANo TADA mRNA could be detectedNANANADirect Sequencing of PCR Products0.00%UneditedAbsent19602623
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products50.00%MediumNone19602623
Agri-140-69-2-CATMA1a58100RNAi (Suppression)RNAiNAPartial TADA suppressionNANANADirect Sequencing of PCR ProductsNAPoorDecreased19602623
Last update: Jul 2021 (version 1.0)