| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
mttB
|
144 |
CDS |
C-to-U |
UUC=>UUU
NA=>NA |
F=>F
NA=>NA |
Synonymous
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | slo2 | NA | NA | NA | Plant growth retardation,delayed development; Sugardependent phenotype | NA | NA | SNaPshot technology | NA | NA | Changed | 22902704 | | Wassilewskija | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 4 weeks | Direct Sequencing of PCR Products | 30.00% | Low | None | 22540321 | | SALK_521900 | slo2-2 | T-DNA Insertion | T-DNA is inserted at 878 bp downstream of the start codon | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22540321 | | Tilling line T94087 | slo2-3 | EMS Mutant | (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83 | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22540321 | | Col-0 | WT | No treatment | No treatment | No treatment | NA | NA | NA | RNA-seq | 28.24% | Low | None | 32098170 | | Col-0 | WT | No treatment | No treatment | No treatment | Normal | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 37.00% | Low | None | 28761003 | | Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 0.00% | Unedited | Absent | 28761003 | | Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 0.00% | Unedited | Absent | 28761003 |
|
| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
mttB
|
145 |
CDS |
C-to-U |
CCG=>UCG
NA=>NA |
P=>S
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | slo2 | NA | NA | NA | Plant growth retardation,delayed development; Sugardependent phenotype | NA | NA | SNaPshot technology | 0.00% | Unedited | Absent | 22902704 | | Wassilewskija | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 4 weeks | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22540321 | | SALK_521900 | slo2-2 | T-DNA Insertion | T-DNA is inserted at 878 bp downstream of the start codon | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22540321 | | Tilling line T94088 | slo2-3 | EMS Mutant | (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83 | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22540321 | | Col-0 | WT | No treatment | No treatment | No treatment | NA | NA | NA | RNA-seq | 90.31% | High | None | 32098170 | | Col-0 | WT | No treatment | No treatment | No treatment | Normal | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 94.00% | High | None | 28761003 | | Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 0.00% | Unedited | Absent | 28761003 | | Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 0.00% | Unedited | Absent | 28761003 |
|
| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
mttB
|
666 |
CDS |
C-to-U |
UCC/UCC=>UCU/UUU
NA=>NA |
S=>S/F
NA=>NA |
Synonymous/Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | slo2 | NA | NA | NA | Plant growth retardation,delayed development; Sugardependent phenotype | NA | NA | SNaPshot technology | NA | NA | Changed | 22902704 | | Wassilewskija | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 4 weeks | Direct Sequencing of PCR Products | 50.00% | Medium | None | 22540321 | | SALK_521900 | slo2-2 | T-DNA Insertion | T-DNA is inserted at 878 bp downstream of the start codon | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 20.00% | Low | Decreased | 22540321 | | Tilling line T94089 | slo2-3 | EMS Mutant | (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83 | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 10.00% | Poor | Decreased | 22540321 | | Col-0 | WT | No treatment | No treatment | No treatment | NA | NA | NA | RNA-seq | 72.48% | High | None | 32098170 | | Col-0 | WT | No treatment | No treatment | No treatment | Normal | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 57.00% | Medium | None | 28761003 | | Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 20.00% | Low | Decreased | 28761003 | | Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 2.00% | Poor | Decreased | 28761003 |
|
| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
nad1
|
2 |
CDS |
C-to-U |
ACG=>AUG
NA=>NA |
T=>M
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | slo2 | NA | NA | NA | Plant growth retardation,delayed development; Sugardependent phenotype | NA | NA | SNaPshot technology | NA | NA | Changed | 22902704 | | Wassilewskija | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 4 weeks | Direct Sequencing of PCR Products | 90.00% | High | None | 22540321 | | SALK_521900 | slo2-2 | T-DNA Insertion | T-DNA is inserted at 878 bp downstream of the start codon | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 55.00% | Medium | Decreased | 22540321 | | Tilling line T94093 | slo2-3 | EMS Mutant | (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83 | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 65.00% | High | Decreased | 22540321 | | Col-0 | WT | No treatment | No treatment | No treatment | Normal | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 100.00% | Complete | None | 28761003 | | Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 4.62% | Poor | Decreased | 28761003 | | Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 0.00% | Unedited | Absent | 28761003 |
|
| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
nad1
|
40 |
CDS |
C-to-U |
CUA=>UUA |
L=>L |
Synonymous |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | slo2 | NA | NA | NA | Plant growth retardation,delayed development; Sugardependent phenotype | NA | NA | SNaPshot technology | NA | NA | Changed | 22902704 | | Wassilewskija | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 4 weeks | Direct Sequencing of PCR Products | 25.00% | Low | None | 22540321 | | SALK_521900 | slo2-2 | T-DNA Insertion | T-DNA is inserted at 878 bp downstream of the start codon | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 22540321 | | Tilling line T94090 | slo2-3 | EMS Mutant | (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83 | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 50.00% | Medium | Decreased | 22540321 |
|
| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
nad1
|
743 |
CDS |
C-to-U |
NA=>NA
CCA=>CUA |
NA=>NA
P=>L |
NA
Recoding |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Col-0 | WT | No treatment | No treatment | No treatment | Normal | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 100.00% | Complete | None | 28761003 | | Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 32.00% | Low | Decreased | 28761003 | | Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 7.00% | Poor | Decreased | 28761003 | | Col-0 | WT | No treatment | No treatment | No treatment | Normal | Seedling | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 100.00% | High | None | 28761003 | | Col-0 | slo2-2 | A T-DNA Insertion | A T‐DNA insertion at 878 bp downstream of the start codon. | NA | When wild‐type seedlings were 3 weeks old, Smaller than WT. | Seedling | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 0.00% | Unedited | Absent | 28761003 | | Col-0 | slo2-3 | A T-DNA Insertion | A third allele, slo2‐3 (Tilling T94087), harbors an ethyl methanesulfonate (EMS)‐induced point mutation (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83. | NA | When wild‐type seedlings were 2 weeks old, the root was smaller than WT's. When wild‐type seedlings were 3 weeks old, Smaller than WT. | Seedling | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 0.00% | Unedited | Absent | 28761003 | | Col-0 | Complemented slo2-2 | Complemented | Performing complementation experiments for slo2‐2 using the full‐length cDNA fused to GFP driven by its native promoter (PSLO2:SLO2‐GFP) and the CaMV‐35S promoter (P35S:SLO2‐GFP). | NA | Reversal of the delay in leaf emergence and suppressed the reduced growth phenotypes in root and shoot | Seedling | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 50.00% | Medium | Decreased | 28761003 | | Col-0 | Complemented slo2-3 | Complemented | NA | NA | NA | Seedling | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 75.00% | High | Decreased | 28761003 |
|
| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
nad4L
|
110 |
CDS |
C-to-U |
UCA=>UUA
NA=>NA |
S=>L
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | slo2 | NA | NA | NA | Plant growth retardation,delayed development; Sugardependent phenotype | NA | NA | SNaPshot technology | 0.00% | Unedited | Absent | 22902704 | | Wassilewskija | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 4 weeks | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22540321 | | SALK_521900 | slo2-2 | T-DNA Insertion | T-DNA is inserted at 878 bp downstream of the start codon | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22540321 | | Tilling line T94091 | slo2-3 | EMS Mutant | (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83 | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22540321 | | Col-0 | WT | No treatment | No treatment | No treatment | NA | NA | NA | RNA-seq | 92.72% | High | None | 32098170 | | Col-0 | WT | No treatment | No treatment | No treatment | Normal | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 95.00% | High | None | 28761003 | | Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 16.00% | Poor | Decreased | 28761003 | | Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 5.00% | Poor | Decreased | 28761003 |
|
| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
nad7
|
739 |
CDS |
C-to-U |
CUU=>UUU
NA=>NA |
L=>F
NA=>NA |
Recoding
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| NA | slo2 | NA | NA | NA | Plant growth retardation,delayed development; Sugardependent phenotype | NA | NA | SNaPshot technology | 0.00% | Unedited | Absent | 22902704 | | Wassilewskija | WT | No treatment | No treatment | No treatment | No treatment | Leaf | 4 weeks | Direct Sequencing of PCR Products | 100.00% | Complete | None | 22540321 | | SALK_521900 | slo2-2 | T-DNA Insertion | T-DNA is inserted at 878 bp downstream of the start codon | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22540321 | | Tilling line T94092 | slo2-3 | EMS Mutant | (C to T) at position 247 downstream of ATG, causing a stop codon at amino acid 83 | NA | Growth retardation( retarded leaf emergence, restricted root growth, and late flowering) | Leaf | 4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 22540321 | | Col-0 | WT | No treatment | No treatment | No treatment | NA | NA | NA | RNA-seq | 96.82% | High | None | 32098170 | | Col-0 | WT | No treatment | No treatment | No treatment | Normal | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 99.00% | High | None | 28761003 | | Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 6.00% | Poor | Decreased | 28761003 | | Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Rosette leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 1.00% | Poor | Decreased | 28761003 |
|
| Arabidopsis thaliana |
AT2G13600 |
Mitochondrion |
nad9
|
92 |
CDS |
C-to-U |
NA=>NA |
NA=>NA |
NA |
|
Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Col-0 | WT | No treatment | No treatment | No treatment | NA | NA | NA | RNA-seq | 98.04% | High | None | 32098170 |
|