PPO1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: PPO1
Synonym: HEMG1, PPOX
Description: Encodes protoporphyrinogen IX Oxidase 1 (PPO1), an enzyme that catalyzes protoporphyrinogen IX into protoporphyrin IX in the tetrapyrrole biosynthetic pathway, plays an important role in editing multiple sites of plastid RNA transcripts, most of which encode subunits of the NADH dehydrogenase-like complex (NDH), in Arabidopsis thaliana.
Protein Family: Protoporphyrinogen oxidase
Subclass: NA
Construct Structure: NA
Gene ID & Species: AT4G01690 (Arabidopsis thaliana)
Edited Gene(s): ndhB    ndhD    rpl23    rpoC1    ndhF    ndhG    petL
Editing Type(s): C-to-U (54)
Publication(s): [1] Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing, PNAS, 2014. [PMID=24497494]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G01690 Chloroplast ndhB 149 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products70.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products60.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products95.00%HighNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhB 467 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products45.00%MediumDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products45.00%MediumDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products90.00%HighNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhB 586 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products70.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products60.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products85.00%HighNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhB 830 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products80.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products70.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhB 836 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products60.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products50.00%MediumDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products95.00%HighNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhB 872 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products85.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products75.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products90.00%HighNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhB 1255 CDS C-to-U CAU=>UAU H=>Y Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products60.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products50.00%MediumDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhB 1481 CDS C-to-U CCA=>CUA P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products75.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products70.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhD 2 CDS C-to-U ACG=>AUG T=>M Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products0.00%UneditedAbsent24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products0.00%UneditedAbsent24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products50.00%MediumNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhD 383 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products65.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products80.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhD 674 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products65.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products90.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhD 878 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products45.00%MediumDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products40.00%MediumDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products95.00%HighNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhD 887 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products65.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products75.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products90.00%HighNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhF 290 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products80.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products80.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast ndhG 50 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products85.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products80.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products100.00%CompleteNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast petL 5 CDS C-to-U CCU=>CUU P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products65.00%HighDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products65.00%HighDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products80.00%HighNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast rpl23 89 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products10.00%PoorDecreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products15.00%PoorDecreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products55.00%MediumNone24497494
Arabidopsis thaliana AT4G01690 Chloroplast rpoC1 488 CDS C-to-U UCA=>UUA S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Salk_143057ppo1-1T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products75.00%HighIncreased24497494
Salk_017634ppo1-2T-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutationEtiolated cotyledons and eventually, seedling lethalityNANADirect Sequencing of PCR Products75.00%HighIncreased24497494
NAWTNo treatmentNo treatmentNo treatmentNo treatmentNANADirect Sequencing of PCR Products30.00%LowNone24497494
Last update: Jul 2021 (version 1.0)