ORRM6 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: ORRM6
Synonym: ORGANELLE RNA RECOGNITION MOTIF PROTEIN 6
Description: Encodes an organelle RNA recognition motif protein required for photosystem II subunit psbF transcript editing.
Protein Family: ORRM
Subclass: NA,ORRM
Construct Structure: RRM, PTP-ORRM
Gene ID & Species: AT1G73530 (Arabidopsis thaliana)
Edited Gene(s): psbF    accD    clpP    matK    ndhB    ndhD    ndhG    rpoA    rpoB    rps12    rps14    atpF    ndhF    petL    psbE    psbZ    rpl23    rpoC1
Editing Type(s): C-to-U (90)
Publication(s): [1] Two organelle RNA recognition motif proteins affect distinct sets of RNA editing sites in the Arabidopsis thaliana plastid, Plant Direct, 2020. [PMID=32259001]
[2] Whole-Transcriptome RNA-seq, Gene Set Enrichment Pathway Analysis, and Exon Coverage Analysis of Two Plastid RNA Editing Mutants, Plant Signaling & Behavior, 2017. [PMID=28387567]
[3] An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing, Plant Physiology, 2017. [PMID=28213559]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G73530 Chloroplast
Plastid
accD 794 CDS C-to-U UCG=>UUG
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SAIL_763_A05orrm6-1T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf4 weeksSanger sequencingNALowDecreased28213559
SAIL_763_A05orrm6-1T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf4 weeksPoisoned Primer Extension (PPE) AssayNANADecreased28213559
WiscDsLox485-488P23orrm6-2T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf5 weeksSTS-PCRseqNALowDecreased28213559
WiscDsLox485-488P23orrm6-2T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf4 weeksSanger sequencingNALowDecreased28213559
WiscDsLox485-488P23orrm6-2T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf4 weeksPoisoned Primer Extension (PPE) AssayNANADecreased28213559
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf5 weeksSTS-PCRseq100.00%CompleteNone28213559
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf4 weeksSanger sequencing100.00%CompleteNone28213559
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf4 weeksPoisoned Primer Extension (PPE) Assay100.00%CompleteNone28213559
SAIL_763_A05orrm6-1T-DNA InsertionT-DNA insertions in the first intron of the ORRM7 geneKnockout; T-DNA insertions in the first intron of the ORRM6 gene result in a truncated ORRM6 transcript lacking exons 2-4Reduced photosystem II (PSII) activity and smaller plant and leaf sizesLeafNASanger sequencing30.70%LowDecreased28387567
WiscDsLox485-488P23orrm6-2T-DNA InsertionT-DNA insertions in the first intron of the ORRM10 geneKnockout; T-DNA insertions in the first intron of the ORRM6 gene result in a truncated ORRM6 transcript lacking exons 2-4Reduced photosystem II (PSII) activity and smaller plant and leaf sizesLeafNASanger sequencing29.00%LowDecreased28387567
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNASanger sequencing85.80%HighNone28387567
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing27.27%LowDecreased32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing29.41%LowDecreased32259001
Arabidopsis thaliana AT1G73530 Plastid accD 1568 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing76.92%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing74.07%HighSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid atpF 92 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid clpP 559 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid matK 640 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing86.96%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing90.48%HighSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 149 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 467 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 586 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 746 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 830 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 836 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 872 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 1255 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhB 1481 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhD 2 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing50.00%MediumSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing62.50%HighIncreased32259001
Arabidopsis thaliana AT1G73530 Plastid ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhD 674 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteIncreased32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteIncreased32259001
Arabidopsis thaliana AT1G73530 Plastid ndhD 878 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhD 887 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhF 290 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid ndhG 50 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid petL 5 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing80.00%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing88.89%HighSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid psbE 214 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Chloroplast
Plastid
psbF 77 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SAIL_763_A05orrm6-1T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf4 weeksSanger sequencing0.00%UneditedAbsent28213559
SAIL_763_A05orrm6-1T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf4 weeksPoisoned Primer Extension (PPE) Assay0.00%UneditedAbsent28213559
WiscDsLox485-488P23orrm6-2T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf5 weeksSTS-PCRseqNAPoorDecreased28213559
WiscDsLox485-488P23orrm6-2T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf4 weeksSanger sequencing0.00%UneditedAbsent28213559
WiscDsLox485-488P23orrm6-2T-DNA InsertionT-DNA is inserted in the first intronKnockout; HomozygousPale green pigmentation, smaller leaf and plant sizes, and retarded growthLeaf4 weeksPoisoned Primer Extension (PPE) Assay0.00%UneditedAbsent28213559
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf5 weeksSTS-PCRseq100.00%CompleteNone28213559
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf4 weeksSanger sequencing100.00%CompleteNone28213559
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeaf4 weeksPoisoned Primer Extension (PPE) Assay100.00%CompleteNone28213559
SAIL_763_A05orrm6-1T-DNA InsertionT-DNA insertions in the first intron of the ORRM6 geneKnockout; T-DNA insertions in the first intron of the ORRM6 gene result in a truncated ORRM6 transcript lacking exons 2-4Reduced photosystem II (PSII) activity and smaller plant and leaf sizesLeafNASanger sequencing6.80%PoorDecreased28387567
WiscDsLox485-488P23orrm6-2T-DNA InsertionT-DNA insertions in the first intron of the ORRM9 geneKnockout; T-DNA insertions in the first intron of the ORRM6 gene result in a truncated ORRM6 transcript lacking exons 2-4Reduced photosystem II (PSII) activity and smaller plant and leaf sizesLeafNASanger sequencing6.30%PoorDecreased28387567
NAWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNASanger sequencing97.10%HighNone28387567
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing0.00%UneditedAbsent32259001
Arabidopsis thaliana AT1G73530 Plastid psbZ 50 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing94.12%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing89.47%HighSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rpl23 89 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing13.33%PoorSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing14.29%PoorSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rpoA 200 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing90.00%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing90.00%HighSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rpoB 338 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing95.00%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing95.00%HighSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rpoB 551 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing100.00%CompleteSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rpoB 2432 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing90.91%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing83.33%HighSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rpoC1 488 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing39.13%LowSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing39.13%LowSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rps12 null Intronic C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing15.00%PoorSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing15.00%PoorSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rps14 80 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing91.67%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing90.91%HighSimilar32259001
Arabidopsis thaliana AT1G73530 Plastid rps14 149 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Columbiaorrm6-1T‐DNA insertion lines orrm6‐1 (SAIL_763_A05)NAHomozygousThe orrm6‐1 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing77.78%HighSimilar32259001
Columbiaorrm6-2T‐DNA insertion lines orrm6‐2 (WiscDsLox485‐488P23)NAHomozygousThe orrm6‐2 mutants were substantially smaller than the wild type, and they displayed reduced PSII photochemical efficiency, small and pale green leaves, and stunted growth.Rosette leaf4-weekRT‐PCR and Sanger sequencing75.00%HighSimilar32259001
Last update: Jul 2021 (version 1.0)