MEF3 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF3
Synonym: MITOCHONDRIAL EDITING FACTOR 3
Description: Encodes a sequence-specific RNA editing factor involved in editing of the atp4-89 site in plant mitochondria.
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: AT1G06140 (Arabidopsis thaliana)
Edited Gene(s): atp4
Editing Type(s): C-to-U (9)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The E-Class PPR Protein MEF3 of Arabidopsis thaliana Can Also Function in Mitochondrial RNA Editing With an Additional DYW Domain, Plant Cell Physiology, 2012. [PMID=22186180]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G06140 Mitochondrion atp4 89 CDS C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22186180
LerWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products50.00%MediumNone22186180
C24WTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22186180
NössenWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNone22186180
Nössenmef3-1T-DNA InsertionT-DNA is inserted in repeat 10NANALeafNADirect Sequencing of PCR Products0.00%UneditedAbsent22186180
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.21%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract93.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract92.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)