MEF20 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF20
Synonym: MITOCHONDRIAL EDITING FACTOR 20
Description: Encodes a pentatricopeptide repeat protein (PPR) protein involved in mitochondrial mRNA editing.
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E
Gene ID & Species: AT3G18970 (Arabidopsis thaliana)
Edited Gene(s): rps4
Editing Type(s): C-to-U (6)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] Reverse Genetic Screening Identifies Five E-class PPR Proteins Involved in RNA Editing in Mitochondria of Arabidopsis thaliana, The Journal of Biological Chemistry, 2010. [PMID=20566637]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G18970 Mitochondrion rps4 226 CDS C-to-U CCC=>UCC
NA=>NA
P=>S
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_051287Cmef20T-DNA InsertionT-DNA is inserted in the CDSHomozygousNo gross disturbance in their growth or development patternsLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent20566637
SALK_051287Cmef20ComplementationIntroduction of the Col wild type version of the gene MEF20 into the mutant protoplastsNANAProtoplastNADirect Sequencing of PCR ProductsNAHighRestored20566637
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq90.50%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract97.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)