Plant Editosome Database
a curated database of RNA editosome in plants

CRR21 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: CRR21
Synonym: CHLORORESPIRATORY REDUCTION 21
Description: Encodes a member of the E+ subgroup of the PPR protein family, containing the E and E+ motifs following a tandem array of PPR motifs. CRR21 is required for editing ndhD in the chloroplast and the PG box of CRR21 is essential for editing.
Protein Family: PPR
Subclass: E+
Construct Structure: PLS-E-E+
Gene ID & Species: JF794491 (Aethionema cordifolium)
NA (Aethionema grandiflorum)
AT5G55740 (Arabidopsis thaliana)
JF794492 (Arabis hirsuta)
JF794507 (Barbarea verna)
JF794493 (Brassica oleracea)
JF794494 (Brassica rapa)
pastoris (Capsella bursa)
JF794496 (Crucihimalaya wallichii)
JF794497 (Draba nemorosa)
NA (Hesperis matronalis)
NA (Iberis amara)
NA (Isatis tinctoria)
JF794498 (Lepidium sativum)
JF794499 (Lepidium virginicum)
JF794508 (Lobularia maritima)
JF794503 (Matthiola incana)
JF794502 (Nasturtium officinale)
JF794500 (Olimarabidopsis pumila)
JF794501 (Raphanus sativus)
JF794506 (Thlaspi arvense)
Edited Gene(s): ndhD
Editing Type(s): C-to-U (30)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Quantitative analysis of motifs contributing to the interaction between PLS-subfamily members and their target RNA sequences in plastid RNA editing, Plant J, 2014. [PMID=25279799]
[3] Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains, The Journal of Biological Chemistry, 2013. [PMID=24194514]
[4] Molecular evolution of pentatricopeptide repeat genes reveals truncation in species lacking an editing target and structural domains under distinct selective pressures, BMC Evol Biol, 2012. [PMID=22583633]
[5] Conserved domain structure of pentatricopeptide repeat proteins involved in chloroplast RNAediting, PNAS, 2007. [PMID=17483454]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Aethionema cordifolium JF794491 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Aethionema grandiflorum NA Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Arabidopsis thaliana AT5G55740 Plastid ndhD null NA C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANo treatmentNANANANANANANA25279799
Arabidopsis thaliana AT5G55740 Chloroplast
Plastid		 ndhD 383 CDS C-to-U UCA=>UUA
NA=>NA		 S=>L
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0crr21-3T-DNA InsertionThe T-DNA insertion in crr21-3 is immediately upstream of the PG box and truncates CRR21 with loss of the PG boxNANANANABulk sequencingNAUneditedAbsent24194514
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentNANABulk sequencingNACompleteNone24194514
crr21-1Ds insertionDs is inserted in the CDS regionHeterozygousDefective NDH activityLeaf3 weeksDirect Sequencing of RT-PCR Products0.00%UneditedAbsent17483454
crr21-2Ds insertionDs is inserted in the CDS regionHeterozygousDefective NDH activityLeaf3 weeksDirect Sequencing of RT-PCR Products0.00%UneditedAbsent17483454
WTNo treatmentNo treatmentNo treatmentNo treatmentLeaf3 weeksDirect Sequencing of RT-PCR Products99.00%HighNone17483454
crr21-1+CRR21ComplementationWild-type genomic DNA covering the sequence encoding At5g55740 is introduced into crr21-1NARestored the transient increase in chlorophyll fluorescence after AL illuminationLeaf3 weeksDirect Sequencing of RT-PCR Products97.00%HighRestored17483454
crr21-2+CRR21ComplementationWild-type genomic DNA covering the sequence encoding At5g55740 is introduced into crr21-2NARestored the transient increase in chlorophyll fluorescence after AL illuminationLeaf3 weeksDirect Sequencing of RT-PCR Products100.00%CompleteRestored17483454
Col-0NANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq96.67%HighNone32098170
Arabis hirsuta JF794492 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SJO02300NANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Barbarea verna JF794507 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing0.00%UneditedNA22583633
Brassica oleracea JF794493 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Brassica rapa JF794494 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Capsella bursa-pastoris JF794495 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Serial # 0051714NANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Crucihimalaya wallichii JF794496 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SJS00500NANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Draba nemorosa JF794497 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SJO02100NANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Hesperis matronalis NA Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
PI 586611NANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Iberis amara NA Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
PI 633243NANANANANALeafNAPCR amplification and bulk sequencing90.00%HighNA22583633
Isatis tinctoria NA Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Lepidium sativum JF794498 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Lepidium virginicum JF794499 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SJO02600NANANANANALeafNAPCR amplification and bulk sequencing90.00%HighNA22583633
Lobularia maritima JF794508 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing0.00%UneditedNA22583633
Matthiola incana JF794503 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing0.00%UneditedNA22583633
Nasturtium officinale JF794502 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Serial #0200327NANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Olimarabidopsis pumila JF794500 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SJS00200NANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Raphanus sativus JF794501 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NANANANANANALeafNAPCR amplification and bulk sequencing100.00%CompleteNA22583633
Thlaspi arvense JF794506 Chloroplast ndhD 383 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Ames 29531NANANANANALeafNAPCR amplification and bulk sequencing0.00%UneditedNA22583633
Last update: Jul 2021 (version 1.0)