AHG11 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: AHG11
Synonym: ABA HYPERSENSITIVE GERMINATION 11
Description: Encodes an E+ PLS family protein that plays a role in mRNA processing in mitochondria, particularly in RNA editing. It is required for germination or early seedling growth stages. Mutant of AHG11 show a weak ABA hypersensitive phenotype.
Protein Family: PPR
Subclass: E+
Construct Structure: PLS-E+
Gene ID & Species: AT2G44880 (Arabidopsis thaliana)
Edited Gene(s): nad4
Editing Type(s): C-to-U (6)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning, Journal of Experimental Botany, 2012. [PMID=22058406]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT2G44880 Mitochondrion nad4 376 CDS C-to-U CGU=>UGU
NA=>NA
R=>C
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
the aba2-1 mutationahg11EMS MutantNonsense mutation [Trp 338 (TGG) -> stop codon (TAG)] results in a lack of 4 C-terminal PPR repeats, E, and E+ motifsNAReduced endogenous ABA levels; The germination levels are slightly lowerSeedlingNADirect Sequencing of PCR Products0.00%UneditedAbsent22058406
NTWTNo treatmentNo treatmentNo treatmentNo treatmentSeedlingNADirect Sequencing of PCR Products100.00%CompleteNone22058406
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.58%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract46.00%MediumDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract31.00%LowDecreased28761003
Last update: Jul 2021 (version 1.0)