Experiment information
Accession CRX101795
Organism Bacteria
Title H08
BioProject PRJCA002202
BioSample SAMC149768
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
whole-BALF sample was used to extract RNA using Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA) and Trizol LS (Thermo Fisher Scientific, Carlsbad, CA, USA) in biosafety II laboratory. The RNA was then reverse transcribed, and amplified using an Ovation Trio RNA-Seq library preparation kit (NuGEN, CA, USA) and was sequenced on an Illumina HiSeq 2500/4000 platform (Illumina, United Kingdom). Reads that were properly mapped to the human reference genome (GRCh38) were discarded (bowtie2 -un/-un-conc). RNA-Seq METATRANSCRIPTOMIC RT-PCR PAIRED
Processing Planned read length (bp) for mate 1: 151
Planned read length (bp) for mate 2: 151
Insert size (bp): 250
Release date2020-03-26
Run
Run accession Run data file information
File nameFile size (MB)
CRR125957 CRR125957_f1.fq.gz
CRR125957_r2.fq.gz
536.14
556.31
SubmitterLili Ren (renliliipb@163.com)
OrganizationInstitute of pathogen Biology
Date submitted2020-03-22