| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
whole-BALF sample was used to extract RNA using Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA) and Trizol LS (Thermo Fisher Scientific, Carlsbad, CA, USA) in biosafety III laboratory. The RNA was then reverse transcribed, and amplified using an Ovation Trio RNA-Seq library preparation kit (NuGEN, CA, USA) and was sequenced on an Illumina HiSeq 2500/4000 platform (Illumina, United Kingdom). Reads that were properly mapped to the human reference genome (GRCh38) were discarded (bowtie2 -un/-un-conc).
|
RNA-Seq |
METATRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
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