Experiment information
Accession CRX020495
Organism Homo sapiens
Title human siOCT4#1_8cell_RNA-seq
BioProject PRJCA000484
BioSample SAMC017361
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
human siOCT4#1_8cell_RNA-seq The embryos were lysed directly and used for cDNA synthesis using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, 634888). The cDNA samples were fragmented to 200-400bp by Covaris sonicator (Covaris). Sequencing libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2018-03-09
Run
Run accession Run data file information
File nameFile size (MB)
CRR024477 CRR024477_f1.fq.gz
CRR024477_r2.fq.gz
5,427.8
5,565.35
SubmitterLei Gao (gaol@big.ac.cn)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2017-11-12