SWISS-2DPAGE Edit

Citations: 48

z-index: 2.4

Basic information
Short name SWISS-2DPAGE
Full name Two-dimensional polyacrylamide gel electrophoresis database
Description SWISS-2DPAGE contains data on proteins identified on various 2-D PAGE and SDS-PAGE reference maps.
URL http://www.expasy.ch/ch2d/ch2d-top
Year founded 1998
Last update & version 2011 3.10.2
Availability Free to academic users only
Contact information
University/Institution hosted Geneva University Hospital
Address
City
Province/State
Country/Region Switzerland
Contact name L Tonella
Contact email luisa.tonella@dim.hcuge.ch
Data information
Object(s)
  • Fungi
Data type(s)
  • Protein
Data Category/Categories
  • Expression
Major organism(s)
  • Escherichia coli
Keyword(s)
  • proteins identified
  • 2-D PAGE
  • SDS-PAGE
Publications
  • Escherichia coli SWISS-2DPAGE database update[PMID: 9740056]
    L Tonella, B J Walsh, J C Sanchez, K Ou, M R Wilkins, M Tyler, S Frutiger, A A Gooley, I Pescaru, R D Appel, J X Yan, A Bairoch, C Hoogland, F S Morch, G J Hughes, K L Williams, D F Hochstrasser,

    The combination of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), computer image analysis and several protein identification techniques allowed the Escherichia coli SWISS-2DPAGE database to be established. This is part of the ExPASy molecular biology server accessible through the WWW at the URL address http://www.expasy.ch/ch2d/ch2d-top.html . Here we report recent progress in the development of the E. coli SWISS-2DPAGE database. Proteins were separated with immobilized pH gradients in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. To increase the resolution of the separation and thus the number of identified proteins, a variety of wide and narrow range immobilized pH gradients were used in the first dimension. Micropreparative gels were electroblotted onto polyvinylidene difluoride membranes and spots were visualized by amido black staining. Protein identification techniques such as amino acid composition analysis, gel comparison and microsequencing were used, as well as a recently described Edman "sequence tag" approach. Some of the above identification techniques were coupled with database searching tools. Currently 231 polypeptides are identified on the E. coli SWISS-2DPAGE map: 64 have been identified by N-terminal microsequencing, 39 by amino acid composition, and 82 by sequence tag. Of 153 proteins putatively identified by gel comparison, 65 have been confirmed. Many proteins have been identified using more than one technique. Faster progress in the E. coli proteome project will now be possible with advances in biochemical methodology and with the completion of the entire E. coli genome.

    Electrophoresis 1998:19(11)

    48 Citations (from Europe PMC, 2018-10-05)

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Record metadata

  • Created on: 2018-02-08
    • Pan Mengyu [2018-09-26]
    • yang zhang [2018-02-24]
    • Ma Lina [2018-02-07]

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