Accession SAMC195286
Sample name mESC_Tet1s ChIP-seq rep2
Title mESC_Tet1s ChIP-seq rep2
Sample type Model organism or animal sample
Organism Mus musculus
Description Mouse ES cells were cultured on gelatin-coated dish using standard protocol. Cells were transfected with OE construct. Cells were crosslinked and crude nuclei were purified. Chromatin was fragmented and subjected to ChIP. DNA was purified and subjected to TruSeq lib prep. The resulted library was size-selected, and subjected to sequencing. Details are in the manuscript.
Attributes
Breed
Strain R1 129/Sv
Age
Biomaterial provider Wei Xie's lab, Tsinghua University
Sex male
Tissue embryonic stem cell
Birth date
Birth location
Breed history
Breed method
Cell line
Cell subtype
Cell type
Collected by
Culture collection
Death date
Development stage
Disease
Disease stage
Genotype Tet1-/- Tet2-/-
Geographic location
Growth protocol
Health state
Isolation source
Latitude and longitude
Phenotype
Type
Specimen voucher
Storage conditions
Stud book number
Treatment ChIP by Flag, Sigma, Cat. F1804
Release date 2020-06-21
BioProject Accession PRJCA002897
Submitter Weikun  Xia  (weikunxia1128@icloud.com)
Organization Tsinghua University
Submission date 2020-06-21

Sample Data

Resource name Description
GSA (1) -
CRA002839 Isoform switch of TET1 regulates DNA demethylation and mouse development