Accession SAMC089378
Sample name R4-rep2
Title RNA-seq of Streptomyces fungicidicus
Sample type Microbe
Organisms Streptomyces fungicidicus
Description Enduracidin, also known as enramycin, is a lipopeptide antibiotic produced by Streptomyces fungicidicus. It possesses a bactericidal activity against gram-positive bacteria, including a number of drug resistant Gram-positive pathogens such as methicillin-resistant Staphylococcus aureus (MRSA). To increase enduracidin production, the transcriptional pattern of enduracidin biosynthesis gene cluster was analyzed and the expression level of each gene at different fermentation period was determined by the transcriptome analysis. One structural gene (endC) was identified from the enduracidin gene cluster, whose expression level was evidently lower than that of the other structural genes at enduracidin producing stages. The gene was identified to be the rate-limiting gene in enduracidin biosynthesis. Through enhancing the expression level of the specific rate-limiting gene (endC), the whole enduracidin titer was significantly improved.
Attributes
Strain missing
Isolation source soil
Collection date 2014-05-03
Geographic location China:Beijing
Altitude
Biomaterial provider
Collected by
Culture collection
Depth
Environment biome
Genotype
Host
Host tissue sampled
Identified by
Lab host
Latitude and longitude
Mating type
Passage history
Sample size
Serotype
Serovar
Specimen voucher
Temperature
Release Date 2019-08-14
Accession PRJCA001646
Submitter zilong  he  (koheallen@163.com)
Organization Beijing Institute of Genomics, Chinese Academy of Sciences
Submission Date 2019-08-14

Sample Data

Resource Name Description
GSA (1) -
CRA001902 Transcriptome based engineering of Streptomyces fungicidicus for improved enduracidin production
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