Accession PRJCA004225
Title Pathological changes and oxidative stress of hypothalamus-pituitary-gonadal axis in hypothyroid rat
Relevance Medical
Data types Raw sequence reads
Organisms Rattus norvegicus
Description Hypothyroidism is a common endocrine disease in both male and female, it is caused by the deficiency of thyroid hormones and could further lead to significant fertility and sterility as well as other complications. The pathogenesis that hypothyroidism induces the dysfunction of testis or ovary and causes reproductive issues is not clear. In this study we constructed a hypothyroid rat model using 6-propyl-2-thiouracil (PTU), then H&E staining and RNA-sequencing were used to detect the pathological and transcriptome changes. Our results demonstrated the nuclear area values of paraventricular nucleus and tuberal hypothalamus neurons were increased, while the acidophils of pituitary was significantly reduced and the basophils was increased in PTU-induced hypothyroid rat. The differentially expressed genes of hypothalamus, pituitary, thyroid, ovary and testis from hypothyroid rats were identified, different tissues shared similar sex hormone-related terms and oxidative stress-related terms in hypothyroid rats through gene ontology analysis, qPCR validated the differential expression of estrogen-related genes and androgen-related genes. Notably, the systemic multi-organ oxidative stress state in hypothyroid rats was confirmed by the expression of oxidative stress-related genes such as the Txnip. The increased oxidative stress level of multi-organs may be an important cause of pathological changes of hypothalamus-pituitary-gonadal axis, and ultimately lead to reproductive health issues.
Sample scope Multiisolate
Release date 2021-01-10
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) National Key Technologies R&D Program 2016YFC1000200
National Natural Science Foundation of China (NSFC) General Program 81771651
Submitter Zhigang    Xue  (
Organization Tongji University
Submission date 2021-01-09

Project Data

Resource name Description
BioSample (48)  show -
GSA (1) -
CRA003725 Pathological changes and oxidative stress of hypothalamus-pituitary-gonadal axis in hypothyroid rat