Accession

PRJCA004225

Title

Pathological changes and oxidative stress of hypothalamus-pituitary-gonadal axis in hypothyroid rat

Relevance

Medical

Data types

Raw sequence reads

Organisms

Rattus norvegicus

Description

Hypothyroidism is a common endocrine disease in both male and female, it is caused by the deficiency of thyroid hormones and could further lead to significant fertility and sterility as well as other complications. The pathogenesis that hypothyroidism induces the dysfunction of testis or ovary and causes reproductive issues is not clear. In this study we constructed a hypothyroid rat model using 6-propyl-2-thiouracil (PTU), then H&E staining and RNA-sequencing were used to detect the pathological and transcriptome changes. Our results demonstrated the nuclear area values of paraventricular nucleus and tuberal hypothalamus neurons were increased, while the acidophils of pituitary was significantly reduced and the basophils was increased in PTU-induced hypothyroid rat. The differentially expressed genes of hypothalamus, pituitary, thyroid, ovary and testis from hypothyroid rats were identified, different tissues shared similar sex hormone-related terms and oxidative stress-related terms in hypothyroid rats through gene ontology analysis, qPCR validated the differential expression of estrogen-related genes and androgen-related genes. Notably, the systemic multi-organ oxidative stress state in hypothyroid rats was confirmed by the expression of oxidative stress-related genes such as the Txnip. The increased oxidative stress level of multi-organs may be an important cause of pathological changes of hypothalamus-pituitary-gonadal axis, and ultimately lead to reproductive health issues.

Sample scope

Multiisolate

Release date

2021-01-10

Grants

Agency	program	Grant ID	Grant title
Ministry of Science and Technology of the People's Republic of China (MOST)	National Key Technologies R&D Program	2016YFC1000200
National Natural Science Foundation of China (NSFC)	General Program	81771651

Submitter

Zhigang Xue (xuezg@tongji.edu.cn)

Organization

Tongji University

Submission date

2021-01-09

Project Data

Resource name	Description
BioSample (48) show	-
SAMC307825	RNAseq of WT male neonatal thyroid 03
SAMC307824	RNAseq of WT male neonatal thyroid 02
SAMC307823	RNAseq of WT male neonatal thyroid 01
SAMC307822	RNAseq of WT male neonatal testis 03
SAMC307821	RNAseq of WT male neonatal testis 02
SAMC307820	RNAseq of WT male neonatal testis 01
SAMC307819	RNAseq of WT male neonatal pituitary 03
SAMC307818	RNAseq of WT male neonatal pituitary 02
SAMC307817	RNAseq of WT male neonatal pituitary 01
SAMC307816	RNAseq of WT male neonatal hypothalamus 03
SAMC307815	RNAseq of WT male neonatal hypothalamus 02
SAMC307814	RNAseq of WT male neonatal hypothalamus 01
SAMC307813	RNAseq of WT female neonatal thyroid 03
SAMC307812	RNAseq of WT female neonatal thyroid 02
SAMC307811	RNAseq of WT female neonatal thyroid 01
SAMC307810	RNAseq of WT female neonatal pituitary 03
SAMC307809	RNAseq of WT female neonatal pituitary 02
SAMC307808	RNAseq of WT female neonatal pituitary 01
SAMC307807	RNAseq of WT female neonatal ovary 03
SAMC307806	RNAseq of WT female neonatal ovary 02
SAMC307805	RNAseq of WT female neonatal ovary 01
SAMC307804	RNAseq of WT female neonatal hypothalamus 03
SAMC307803	RNAseq of WT female neonatal hypothalamus 02
SAMC307802	RNAseq of WT female neonatal hypothalamus 01
SAMC307801	RNAseq of PTU-induced male neonatal thyroid 05
SAMC307800	RNAseq of PTU-induced male neonatal thyroid 02
SAMC307799	RNAseq of PTU-induced male neonatal thyroid 01
SAMC307798	RNAseq of PTU-induced male neonatal testis 05
SAMC307797	RNAseq of PTU-induced male neonatal testis 02
SAMC307796	RNAseq of PTU-induced male neonatal testis 01
SAMC307795	RNAseq of PTU-induced male neonatal pituitary 05
SAMC307794	RNAseq of PTU-induced male neonatal pituitary 02
SAMC307793	RNAseq of PTU-induced male neonatal pituitary 01
SAMC307792	RNAseq of PTU-induced male neonatal hypothalamus 05
SAMC307791	RNAseq of PTU-induced male neonatal hypothalamus 02
SAMC307790	RNAseq of PTU-induced male neonatal hypothalamus 01
SAMC307789	RNAseq of PTU-induced female neonatal thyroid 06
SAMC307788	RNAseq of PTU-induced female neonatal thyroid 04
SAMC307787	RNAseq of PTU-induced female neonatal thyroid 01
SAMC307786	RNAseq of PTU-induced female neonatal pituitary 06
SAMC307785	RNAseq of PTU-induced female neonatal pituitary 04
SAMC307784	RNAseq of PTU-induced female neonatal pituitary 01
SAMC307783	RNAseq of PTU-induced female neonatal ovary 06
SAMC307782	RNAseq of PTU-induced female neonatal ovary 04
SAMC307781	RNAseq of PTU-induced female neonatal ovary 01
SAMC307780	RNAseq of PTU-induced female neonatal hypothalamus 06
SAMC307779	RNAseq of PTU-induced female neonatal hypothalamus 04
SAMC307778	RNAseq of PTU-induced female neonatal hypothalamus 01
GSA (1)	-
CRA003725	Pathological changes and oxidative stress of hypothalamus-pituitary-gonadal axis in hypothyroid rat