Accession PRJCA003828
Title methylome of Klebsiella pneumoniae
Relevance Medical
Data types Raw sequence reads
Assembly
Organisms Klebsiella pneumoniae
Description Klebsiella pneumoniae ( K. pneumonia ) is an important pathogen that can cause severe hospital-/community-acquired infections. To panoramically analyze K. pneumoniae 's methylation features, we completed the whole genome sequence of 14 K. pneumoniae strains covering various serotypes, MLSTs, CGs, viscosity/virulence and drug-resistances, and further characterized their methylomes using Pacbio-SMRT and Bisulfite technologies. We identified 15 methylated motifs including 13 6mA and two 5mC methylated motifs, among which eight were novel ones and their corresponding MTases were further validated. Additionally, we analyzed the genomic distribution of G A T C and C C W G G methylation motifs shared by all strains, and identified differential distributive patterns with some hemi/un-methylated GATC motifs tending to locate in the intergenic regions (IGRs). Specifically, we characterized the in vivo methylation kinetics at single-base resolution in genome-wide through simulating the dynamic processes of replication-mediated passive demethylation and MTase-catalyzed re-methylation. The slower methylation-rates of the G A T C motifs in the replication origins ( oriC ) and IGRs suggest an epigenetic mechanism implicated in the regulation of replication-initiation and transcription. Our findings illustrate the first comprehensive dynamic methylome map of K. pneumonia at single base resolution, and provide an efficient means and important reference for a better understanding of epigenetic regulation in bacteria.
Sample scope Multiisolate
Release date 2020-11-05
Biomaterial provider State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
Grants
Agency program Grant ID Grant title
No funding support
Submitter Fei    Chen  (chenfei@big.ac.cn)
Organization Beijing Institute of Genomics, Chinese Academy of Sciences
Submission date 2020-11-05

Project Data

Resource name Description
BioSample (24)  show -
GSA (1) -
CRA003482 Precision methylome and in vivo methylation kinetics characterization of Klebsiella pneumoniae