| Accession |
PRJCA003347 |
| Title |
Dietary emulsifiers directly impact adherent-invasive E. coli gene expression to drive chronic intestinal inflammation |
| Relevance |
Medical |
| Data types |
Transcriptome or Gene expression
16S
|
| Organisms |
Escherichia coli
|
| Description |
Dietary emulsifiers carboxymethylcellulose (CMC) and Polysorbate-80 (P80) disturb gut microbiota, promoting chronic inflammation. Mice with minimal microbiota are protected against emulsifiers effects, leading us to hypothesize that these compounds might provoke pathobionts to promote inflammation. Gnotobiotic WT and IL10ko mice were colonized with Crohn disease-associated adherent-invasive E. coli (AIEC) and subsequently administered CMC or P80. AIEC colonization of GF and ASF mice results in chronic intestinal inflammation and metabolism dysregulations when consuming emulsifier. In IL10ko mice, AIEC mono-colonization results in severe intestinal inflammation in response to emulsifiers. Exposure of AIEC to emulsifiers in vitro increase its motility and ability to adhere to intestinal epithelial cells. Transcriptomic analysis reveal that emulsifiers directly induce expression of clusters of genes that mediate AIEC virulence and promotion of inflammation. To conclude, emulsifiers promote virulence and encroachment of pathobionts, providing a means by which these compounds may drive inflammation in hosts carrying such bacteria. |
| Sample scope |
Stool |
| Release date |
2020-10-01 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 33027647
|
Dietary Emulsifiers Directly Impact Adherent-Invasive E. coli Gene Expression to Drive Chronic Intestinal Inflammation
|
Cell Reports
|
10.1016/j.celrep.2020.108229
|
2020
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| Kenneth Rainin Foundation
|
Innovator Award
|
1
|
|
|
|
| Submitter |
Benoit
Chassaing (benoit.chassaing@inserm.fr)
|
| Organization |
INSERM |
| Submission date |
2020-08-29 |
