Accession

PRJCA003192

Title

Gene knocked out in Mycobacterium tuberculosis

Relevance

Medical

Umbrella project

eGPS: evolutionary Genotype-Phenotype Systems biology

Key theories & Model

Special portal and non model biological system

Data types

Whole genome sequencing

Organisms

Mycobacterium tuberculosis

Description

We repurposed an endogenous type III-A CRISPR-Cas to enable programmable genome editing and RNA level knockdown in Mycobacterium tuberculosis (Mtb). The latter CRISPRi tool was also successfully applied to screen out growth-regulation genes for this important human pathogen

Sample scope

Lab cultured

Release date

2020-08-07

Publication

PubMed ID	Article title	Journal name	DOI	Year
34923124	Reprogramming Mycobacterium tuberculosis CRISPR System for Gene Editing and Genome-wide RNA Interference Screening	Genomics, Proteomics & Bioinformatics	10.1016/j.gpb.2021.01.008	2021

Grants

Agency	program	Grant ID	Grant title
National Natural Science Foundation of China (NSFC)	Major Research Plan	C180501	Vaccine searching for TB
Chinese Academy of Sciences (CAS)	Strategic Priority Research Program of Chinese Academy of Sciences (CAS)	XDB13000000

Submitter

khaista rahman (3027224182@qq.com)

Organization

Huazhong Agricultural University

Submission date

2020-08-05

Project Data

Resource name	Description
BioSample (4)	-
SAMC204698	M_KO3
SAMC204697	M_KO2
SAMC204689	M_KO1
SAMC204687	M_Ctrl1
GSA (1)	-
CRA003057	Gene knocked out in Mycobacterium tuberculosis