| Accession |
PRJCA002886 |
| Title |
Arabidopsis Messenger RNA N6-methyladenosine Demethylation Regulates Plant Development |
| Relevance |
Model organism |
| Data types |
Epitranscriptome
|
| Organisms |
Arabidopsis thaliana
|
| Description |
N6-methyladenosine (m6A) is the most abundant internal modification in the messenger RNA (mRNA) of all higher eukaryotes. This modification has been shown to be reversible in mammals; it is installed by a methyltransferase heterodimer complex of METTL3 and METTL14 bound with WTAP, and reversed by iron(II)- and α-ketoglutarate-dependent demethylases FTO and ALKBH5. This modification exhibits significant functional roles in various biological processes. The m6A modification as a RNA mark is recognized by reader proteins, such as YTH domain family proteins and HNRNPA2B1; m6A can also act as a structure switch to affect RNA-protein interactions for biological regulation. In Arabidopsis thaliana, the methyltransferase subunit MTA (the plant orthologue of human METTL3, encoded by At4g10760) was well characterized and FIP37 (the plant orthologue of human WTAP) was first identified as the interacting partner of MTA. Here we report the discovery and characterization of reversible m6A methylation mediated by AtALKBH10B (encoded by At4g02940) in A. thaliana, and noticeable roles of this RNA demethylase in affecting plant development and floral transition. Our findings reveal potential broad functions of reversible mRNA methylation in plants. |
| Sample scope |
Multiisolate |
| Release date |
2020-06-19 |
| Publication |
| PubMed ID |
Article title |
Journal name |
DOI |
Year |
| 29180595
|
|
|
|
|
|
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
21432002
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2016YFC0900302
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2017YFA0505201
|
|
| Ministry of Science and Technology of the People's Republic of China (MOST)
|
|
2014CB964900
|
|
| National Natural Science Foundation of China (NSFC)
|
|
21372022
|
|
| National Natural Science Foundation of China (NSFC)
|
|
21210003
|
|
|
|
| Accessions in other database |
| Accession |
Database name |
| GSE79523
|
NCBI
|
|
|
| Submitter |
Hong-Chao
Duan (duanhc@pku.edu.cn)
|
| Organization |
Peking University |
| Submission date |
2020-06-19 |
