Accession PRJCA002886
Title Arabidopsis Messenger RNA N6-methyladenosine Demethylation Regulates Plant Development
Relevance Model organism
Data types Epitranscriptome
Organisms Arabidopsis thaliana
Description N6-methyladenosine (m6A) is the most abundant internal modification in the messenger RNA (mRNA) of all higher eukaryotes. This modification has been shown to be reversible in mammals; it is installed by a methyltransferase heterodimer complex of METTL3 and METTL14 bound with WTAP, and reversed by iron(II)- and α-ketoglutarate-dependent demethylases FTO and ALKBH5. This modification exhibits significant functional roles in various biological processes. The m6A modification as a RNA mark is recognized by reader proteins, such as YTH domain family proteins and HNRNPA2B1; m6A can also act as a structure switch to affect RNA-protein interactions for biological regulation. In Arabidopsis thaliana, the methyltransferase subunit MTA (the plant orthologue of human METTL3, encoded by At4g10760) was well characterized and FIP37 (the plant orthologue of human WTAP) was first identified as the interacting partner of MTA. Here we report the discovery and characterization of reversible m6A methylation mediated by AtALKBH10B (encoded by At4g02940) in A. thaliana, and noticeable roles of this RNA demethylase in affecting plant development and floral transition. Our findings reveal potential broad functions of reversible mRNA methylation in plants.
Sample scope Multiisolate
Release date 2020-06-19
Publication
PubMed ID Article title Journal name DOI Year
29180595
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 21432002
Ministry of Science and Technology of the People's Republic of China (MOST) 2016YFC0900302
Ministry of Science and Technology of the People's Republic of China (MOST) 2017YFA0505201
Ministry of Science and Technology of the People's Republic of China (MOST) 2014CB964900
National Natural Science Foundation of China (NSFC) 21372022
National Natural Science Foundation of China (NSFC) 21210003
Accessions in other database
Accession Database name
GSE79523 NCBI
Submitter Hong-Chao    Duan  (duanhc@pku.edu.cn)
Organization Peking University
Submission date 2020-06-19

Project Data

Resource name Description
BioSample (4) -
SAMC250389 m6A-seq of Arabidopsis thaliana: wild type col-0 rep1
SAMC250392 m6A-seq of Arabidopsis thaliana: alkbh10b-1 rep2
SAMC250391 m6A-seq of Arabidopsis thaliana: alkbh10b-1 rep1
SAMC250390 m6A-seq of Arabidopsis thaliana: wild type col-0 rep2
GSA (1) -
CRA002826 Arabidopsis Messenger RNA N6-methyladenosine Demethylation Regulates Plant Development