| Accession |
PRJCA002848 |
| Title |
Studying m6A reader ECT2 function in Arabidopsis |
| Relevance |
Agricultural |
| Data types |
Raw sequence reads
|
| Organisms |
Arabidopsis
|
| Description |
In order to understanding the molecular mechanisms of N6-methyladenosine (m6A) in regulating the RNA metabolism and plant development, we identified the first m6A-binding protein ECT2 in Arabidopsis. We developed a high-throughput method FA-CLIP (formaldehyde cross-linking and immuno-precipitation) to map the endogenous RNA substrates of ECT2. We found that ECT2 mainly interacted with 3' untranslated regions (3' UTRs) of mRNAs, and recognized a plant-specific m6A motif UGUA. Our results showed that ECT2 regulated trichome morphology in Arabidopsis through stabilizing several trichome development-related mRNAs. |
| Sample scope |
Multiisolate |
| Release date |
2020-06-14 |
| Publication |
|
| Grants |
| Agency |
program |
Grant ID |
Grant title |
| National Natural Science Foundation of China (NSFC)
|
|
21432002
|
|
| National Natural Science Foundation of China (NSFC)
|
|
21372022
|
|
| National Natural Science Foundation of China (NSFC)
|
|
21210003
|
|
| National Basic Research Program of China
|
|
2017YFA0505201
|
|
| National Basic Research Program of China
|
|
MOST2016YFC0900302
|
|
| National Basic Research Program of China
|
|
2014CB964900
|
|
|
|
| Submitter |
Lianhuan
Wei (weilianhuan@pku.edu.cn)
|
| Organization |
Peking University |
| Submission date |
2020-06-14 |
