Accession PRJCA002841
Title Bisulfite Free Nanoscale Analysis of Hydroxymethylcytosine at Single Base Resolution
Relevance Medical
Data types Epigenomics
Organisms Homo sapiens
Description High-resolution detection of genome-wide 5-hydroxymethylcytosine (5hmC) sites of small-scale samples remains challenging. Here, we present hmC- CATCH, a bisulfite-free, base-resolution method for the genome-wide detection of 5hmC. hmC-CATCH is based on selective 5hmC oxidation, chemical labeling and subsequent C-to-T transition during PCR. Requiring only nanoscale input genomic DNA samples, hmC- CATCH enabled us to detect genome-wide hydroxyme- thylome of human embryonic stem cells in a cost-effective manner. Further application of hmC-CATCH to cell-free DNA (cfDNA) of healthy donors and cancer patients revealed base-resolution hydroxymethylome in the human cfDNA for the first time. We anticipate that our chemical biology approach will find broad applications in hydroxymethylome analysis of limited biological and clinical samples.
Sample scope Multiisolate
Release date 2020-09-03
Publication
PubMed ID Article title Journal name DOI Year
30278133
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 21825701
National Natural Science Foundation of China (NSFC) 91953201
Ministry of Science and Technology of the People 2019YFA0110900
Accessions in other database
Accession Database name
GSE112048 NCBI
Submitter He    Bo  (hebo@pku.edu.cn)
Organization Peking University
Submission date 2020-06-12

Project Data

Resource name Description
BioSample (13)  show -
GSA (1) -
CRA002801 Bisulfite-Free, Nanoscale Analysis of Hydroxymethylcytosine at Single Base Resolution