Accession PRJCA002567
Title Single symbiotic cell transcriptome sequencing of a coral
Relevance Environmental
Data types Transcriptome or Gene expression
Raw sequence reads
Genome sequencing
Organisms coral metagenome
Description Symbiotic zooxanthellae is crucial to coral's health. et we still know little about the mechanism behind the intracellular symbiosis. Here, we first used single-cell transcriptome sequencing technology (sc-RNA-Seq) on coral to explore the symbiotic characters. We successfully detected 9110 single cells. By mapping the RNA-Seq reads to coral and zooxanthellae genomes respectively using Drop-seq core computational protocol (drop-seq-tools-2.3.0, https://github.com/broadinstitute/Drop-seq/releases), 4871 symbiotic cells were identified. Further clustering analysis grouped the 4871 cells into five groups, which are not defined previously.
Sample scope Single cell
Release date 2020-04-22
Grants
Agency program Grant ID Grant title
National Natural Science Foundation of China (NSFC) 81827901
National Natural Science Foundation of China (NSFC) 81830053
National Natural Science Foundation of China (NSFC) 21327902
National Natural Science Foundation of China (NSFC) 61850410528
National Key Research and Development Program of China 2018YFF0215200
National Key Research and Development Program of China 2017YFA0205700
Fundamental Research Project of Shenzhen Science and Technology Innovation Committee JCYJ20180306174552939
Fundamental Research Funds for the Central Universities 2242017K3DN26
Submitter Huamei    Li  (230179525@seu.edu.cn)
Organization Southeast university
Submission date 2020-04-21

Project Data

Resource name Description
BioSample (5) -
SAMC180071 Coral singl-cell transcriptome sequencing_CF14_L4
SAMC180070 Coral singl-cell transcriptome sequencing_CF13_L4
SAMC180069 Coral singl-cell transcriptome sequencing_CF12_L4
SAMC180068 Coral singl-cell transcriptome sequencing_CF11_L4
SAMC180067 Coral singl-cell transcriptome sequencing_CF10_L4
GSA (1) -
CRA002560 Single Symbiotic Cell Transcriptome Sequencing of Coral