Accession |
PRJCA002567 |
Title |
Single symbiotic cell transcriptome sequencing of a coral |
Relevance |
Environmental |
Data types |
Transcriptome or Gene expression
Raw sequence reads
Genome sequencing
|
Organisms |
coral metagenome
|
Description |
Symbiotic zooxanthellae is crucial to coral's health. et we still know little about the mechanism behind the intracellular symbiosis. Here, we first used single-cell transcriptome sequencing technology (sc-RNA-Seq) on coral to explore the symbiotic characters. We successfully detected 9110 single cells. By mapping the RNA-Seq reads to coral and zooxanthellae genomes respectively using Drop-seq core computational protocol (drop-seq-tools-2.3.0, https://github.com/broadinstitute/Drop-seq/releases), 4871 symbiotic cells were identified. Further clustering analysis grouped the 4871 cells into five groups, which are not defined previously. |
Sample scope |
Single cell |
Release date |
2020-04-22 |
Grants |
Agency |
program |
Grant ID |
Grant title |
National Natural Science Foundation of China (NSFC)
|
|
81827901
|
|
National Natural Science Foundation of China (NSFC)
|
|
81830053
|
|
National Natural Science Foundation of China (NSFC)
|
|
21327902
|
|
National Natural Science Foundation of China (NSFC)
|
|
61850410528
|
|
National Key Research and Development Program of China
|
|
2018YFF0215200
|
|
National Key Research and Development Program of China
|
|
2017YFA0205700
|
|
Fundamental Research Project of Shenzhen Science and Technology Innovation Committee
|
|
JCYJ20180306174552939
|
|
Fundamental Research Funds for the Central Universities
|
|
2242017K3DN26
|
|
|
Submitter |
Huamei
Li (230179525@seu.edu.cn)
|
Organization |
Southeast university |
Submission date |
2020-04-21 |