Accession PRJCA002195
Title Domesticated cynomolgus monkey embryonic stem cells
Relevance Model organism
Data types Transcriptome or Gene expression
Organisms Macaca fascicularis
Description Blastocyst complementation by pluripotent stem cell (PSC) injection is believed to be the most promising method to generate xenogeneic organs. However, ethical issues prevent the study of human chimeras in the late embryonic stage of development. Primate embryonic stem cells (ESCs), which have similar pluripotency to human ESCs, are a good model for studying interspecies chimerism and organ generation. However, whether primate ESCs can be used in xenogenous grafts remains unclear. In this study, we evaluated the chimeric ability of cynomolgus monkey (Macaca fascicularis) ESCs (cmESCs) in pigs, which are excellent hosts because of their many similarities to humans. We report an optimized culture medium that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos, in which domesticated cmESCs (D-ESCs) injected into pig blastocysts differentiated into cells of all three germ layers. In addition, we obtained two neonatal interspecies chimeras, in which we observed tissue-specific D-ESC differentiation. Taken together, the results demonstrate the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model, with a chimeric ratio of 0.001-0.0001 in different neonate tissues. We believe this work will facilitate future developments in xenogeneic organogenesis, bringing us one step closer to producing tissue-specific functional cells and organs in a large animal model through interspecies blastocyst complementation.
Sample scope Monoisolate
Release date 2020-02-07
Publication
PubMed ID Article title Journal name DOI Year
31781970 10.1007/s13238-019-00676-8
Grants
Agency program Grant ID Grant title
Chinese Academy of Sciences (CAS) Strategic Priority Research Program of Chinese Academy of Sciences (CAS) XDA16030400
Submitter Guihai    Feng  (fenggh@aliyun.com)
Organization Institute of Zoology, Chinese Academy of Sciences
Submission date 2020-02-07

Project Data

Resource name Description
BioSample (4) -
SAMC134274 cmESC1
SAMC134277 dESC2
SAMC134276 dESC1
SAMC134275 cmESC2
GSA (1) -
CRA002330 Domesticated cynomolgus monkey embryonic stem cells