Accession PRJCA001901
Title The histone modification reader ZCWPW1 links histone methylation to repair of PRDM9-induced meiotic double stand breaks
Relevance Medical
Data types Epigenomics
Raw sequence reads
Organisms Mus musculus
Description It is known that the histone modification writer PRDM9 deposits H3K4me3 and H3K36me3 marks at future DSB sites very early in meiosis, the nature of any proteins which can read such marks is unknown. Here, we demonstrate in vivo that ZCWPW1 is a H3K4me3 reader and show that its binding at chromatin promotes completion of DSB repair and synapsis in mouse testes. Based on multiple ChIP-seq and immunofluorescence analyses with mutants-including an H3K4me3-reader-dead variant of ZCWPW1 mice line-we establish that ZCWPW1's occupancy on chromatin is strongly but not exclusively promoted by the histone-modification activity of the PRDM9. ZCWPW1 localizes to DMC1-labelled DSB hotspots in a largely PRDM9-dependent manner, where it facilitates completion of synapsis by mediating the DSB repair process. In sum, our study demonstrates the function of a reader protein that carries out work resulting from an epigenetics-based recombination hotspot selection system in mammals.
Sample scope Multispecies
Release date 2020-06-20
Publication
PubMed ID Article title Journal name DOI Year
32374261 The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair eLife 10.7554/eLife.53459 2020
Grants
Agency program Grant ID Grant title
Ministry of Science and Technology of the People's Republic of China (MOST) 2018YFC1003400
Submitter Shenli    Yuan  (yuanshenli@big.ac.cn)
Organization Beijing Institute of Genomics, Chinese Academy of Sciences
Submission date 2019-11-11

Project Data

Resource name Description
BioSample (21)  show -
GSA (1) -
CRA002088 The histone modification reader ZCWPW1 links histone methylation to repair of PRDM9-induced meiotic double stand breaks