Accession |
PRJCA001735 |
Title |
Mucosal profiling of pediatric-onset colitis and IBD reveals a common pathogenesis and therapeutic target |
Relevance |
Medical |
Data types |
Transcriptome or Gene expression
|
Organisms |
Homo sapiens
|
Description |
Pediatric-onset colitis and inflammatory bowel disease (IBD) have significant impacts on the growth of infants and children, but the etiopathogenesis underlying disease subtypes remains incompletely understood. Here, we report single-cell clustering, immune phenotyping, and risk gene analysis for children with undifferentiated colitis, Crohn's disease and ulcerative colitis. We not only demonstrated characteristics that was disease-specific, but also pathogenesis that was common and underpinned by the impaired cAMP-response pathway. Specifically, we showed that infiltration of PDE4B- and TNF-expressing macrophages, decreased abundance of CD39-expressing intraepithelial T cells, and platelet aggregation and release of 5-hydroxytryptamine at the colonic mucosae were common in colitis and IBD patients in comparison with control subjects. Targeting these pathways using the phosphodiesterase inhibitor dipyridamole restored immune homeostasis and improved clinical symptoms for children with colitis. In summary, comprehensive analysis of the colonic mucosae has uncovered common pathogenesis and a novel treatment for children with colitis and IBD. |
Sample scope |
Single cell |
Release date |
2019-11-01 |
Publication |
PubMed ID |
Article title |
Journal name |
DOI |
Year |
31730855
|
Mucosal Profiling of Pediatric-Onset Colitis and IBD Reveals Common Pathogenics and Therapeutic Pathways
|
Cell
|
10.1016/j.cell.2019.10.027
|
2019
|
|
Grants |
Agency |
program |
Grant ID |
Grant title |
National Natural Science Foundation of China (NSFC)
|
|
91742109
|
|
|
Submitter |
Yuxia
Zhang (yuxia.zhang@gwcmc.org)
|
Organization |
Guangzhou Women and Children's Medical Center |
Submission date |
2019-09-10 |