Accession |
PRJCA001372 |
Title |
HCC Non-invasive Surveillance by Low-pass Whole Genome-wide Bisulfite Sequencing. |
Relevance |
Medical |
Data types |
Epigenomics
Raw sequence reads
|
Organisms |
Homo sapiens
|
Description |
Circulating cell-free DNA methylation has been demonstrated to be a promising strategy for non-invasive cancer diagnosis. However, low-level of cell-free DNA in plasma and high cost of whole genome bisulfite sequencing (WGBS) limits sequencing depth and subsequent biomarker identification of cell-free DNA in plasma. Here we demonstrate long-region hypo-methylation (LRM) in low-pass WGBS data (<5-million reads) provide high sensitivity and specificity surveillance to hepatocellular carcinoma (HCC). We applied low-pass WGBS approach and demonstrated DNA methylation abnormalities in HCC occurred in the HBV integration regions. These findings reflect the stage of liver disease progression thereby providing a suitable surrogate for methylation level estimation in plasma cfDNA analysis of liver diseases. |
Sample scope |
Multiisolate |
Release date |
2020-04-10 |
Publication |
PubMed ID |
Article title |
Journal name |
DOI |
Year |
32741373
|
Hypomethylation in HBV integration regions aids non-invasive surveillance to hepatocellular carcinoma by low-pass genome-wide bisulfite sequencing
|
BMC Medicine
|
10.1186/s12916-020-01667-x
|
2020
|
|
Grants |
Agency |
program |
Grant ID |
Grant title |
National Natural Science Foundation of China (NSFC)
|
|
81201700
|
|
|
Submitter |
Haikun
Hai
Zhang (zhanghk@big.ac.cn)
|
Organization |
Beijing Institute of Genomics, Chinese Academy of Sciences |
Submission date |
2019-04-12 |